Miranda Michele, Urioste Daniele, Andrade Souza Livia T, Mendes Adriano A, de Castro Heizir F
Engineering School of Lorena, University of São Paulo, P.O. Box 116, 12602-810 Lorena, SP, Brazil.
Enzyme Res. 2011;2011:216435. doi: 10.4061/2011/216435. Epub 2011 Aug 16.
Lipase from Candida rugosa (CRL) was immobilized by covalent attachment on hydrous niobium oxide. The matrix could effectively be attached to the enzyme with high retention of activity and prevent its leakage. Following immobilization, CRL exhibited improved storage stability and performed better at higher incubation temperatures. In addition, the enzyme retained most of its catalytic efficiency after successive operational cycles. The immobilized derivative was also fully characterized with respect to its morphological properties: particle size, surface specific area, and pore size distribution. Structural integrity and conformational changes, such as surface cavities in the support, set by the lipase procedure, were observed by Scanning Electron Microscopy. Additionally, a comparative study between free and immobilized lipases was provided in terms of pH, temperature, and thermal stability. CRL derivative was evaluated for the synthesis of biodiesel employing babassu oil and short chain alcohols. The process was feasible only for oil and butanol reaction system.
来自皱褶假丝酵母的脂肪酶（CRL）通过共价连接固定在水合氧化铌上。该基质能够有效地与酶结合，同时保持高活性，并防止酶泄漏。固定化后，CRL表现出更好的储存稳定性，并且在较高的孵育温度下表现更佳。此外，该酶在连续操作循环后仍保留了大部分催化效率。还对固定化衍生物的形态学性质进行了全面表征：粒径、比表面积和孔径分布。通过扫描电子显微镜观察了由脂肪酶程序设定的结构完整性和构象变化，例如载体中的表面空洞。此外，还对游离脂肪酶和固定化脂肪酶在pH值、温度和热稳定性方面进行了比较研究。对使用巴巴苏油和短链醇合成生物柴油的CRL衍生物进行了评估。该工艺仅对油和丁醇反应体系可行。
