State Key Laboratory of Chemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
Bioresour Technol. 2010 Jun;101(11):3830-7. doi: 10.1016/j.biortech.2010.01.023. Epub 2010 Feb 8.
The influences of pore diameter and cross-linking method on the immobilization efficiency of Candida rugosa lipase (CRL) in a mesoporous material have been investigated. Five kinds of SBA-15 with different pore-sizes (6.8nm, 9.1nm, 13.2nm, 15.6nm and 22.4nm) were chosen as the carrier. The one with pore diameter of 15.6nm was proved to be a more suitable immobilization support than the others, and the loading amount reached 343.6mg/g. To solve the leaching problem of the adsorbed enzyme, chitosan and glutaraldehyde were used as the "bridge unit" and "cross-linking agent", respectively, which were supposed to realize the immobilization of lipase molecules adsorbed on the surface of pores into a mesh-like layer. The experimental results showed that the activities of the immobilized CRL were much higher than that of free lipase and remained 80.5% of the initial activity after 6 cycles in 48h.
研究了孔径和交联方法对固定化脂肪酶（CRL）在介孔材料中固定化效率的影响。选用了五种不同孔径（6.8nm、9.1nm、13.2nm、15.6nm 和 22.4nm）的 SBA-15 作为载体。结果表明，孔径为 15.6nm 的 SBA-15 比其他载体更适合作为固定化支撑材料，其载量达到 343.6mg/g。为了解决吸附酶的浸出问题，使用壳聚糖和戊二醛分别作为“桥联单元”和“交联剂”，将吸附在孔表面的酶分子固定化在网状层中。实验结果表明，固定化 CRL 的活性明显高于游离脂肪酶，在 48 小时内经过 6 次循环后，仍保持初始活性的 80.5%。
