Burke D A, Clayden S A, Axon A T
Gastroenterology Unit, General Infirmary, Leeds.
J Clin Pathol. 1990 Apr;43(4):316-9. doi: 10.1136/jcp.43.4.316.
A microtitre method was developed to screen Escherichia coli from 48 patients with ulcerative colitis and 25 controls for serum resistance. Bactericidal resistance was indicated by a change in colour of indicator due to acid production by viable organisms and quantitated by a change in absorbance. The method clearly differentiated between organisms confirmed as resistant or sensitive by conventional techniques. Twenty four (50%) disease and 14 (56%) control E coli specimens showed serum resistance. Bactericidal competence of sera from patients with ulcerative colitis was assessed by incubating sensitive E coli with sera from 10 patients with ulcerative colitis and pooled normal serum. All sera effectively reduced viable counts to less than 6% of original inoculum. This study shows that serum samples from patients with ulcerative colitis are bactericidally competent and that there is no increase in the number of serum resistant E coli in patients with ulcerative colitis.
开发了一种微量滴定法，用于筛查48例溃疡性结肠炎患者和25例对照者的血清中大肠杆菌的血清抗性。由于活生物体产酸导致指示剂颜色变化表明存在杀菌抗性，并通过吸光度变化进行定量。该方法能够明确区分经传统技术确认为抗性或敏感的生物体。24份（50%）疾病组和14份（56%）对照组大肠杆菌标本显示出血清抗性。通过将敏感大肠杆菌与10例溃疡性结肠炎患者的血清及混合正常血清孵育，评估溃疡性结肠炎患者血清的杀菌能力。所有血清均有效地将活菌数降低至原始接种量的6%以下。本研究表明，溃疡性结肠炎患者的血清样本具有杀菌能力，且溃疡性结肠炎患者中血清抗性大肠杆菌的数量没有增加。
