Thoracic Department, Tianjin Chest Hospital, Tianjin Medical University, Xi'an Road, Tianjin 300051, People's Republic of China.
Dig Dis Sci. 2012 Jan;57(1):32-7. doi: 10.1007/s10620-011-1864-y. Epub 2011 Aug 31.
Esophageal carcinoma is one of the main malignancies in China. Previous studies indicated that matrix metalloproteinases (MMPs) play important roles in the process of tumor invasion and metastasis in several types of solid tumors. Among all of the MMPs, MMP-2 is one of the MMPs closely associated with tumor invasion. In this study, we suppressed MMP-2 expression with RNA interference and then observed inhibitory effects on the invasion and migration of the esophageal carcinoma cell line KYSE150.
Three target sequences were selected and siRNA against MMP-2 mRNA were synthesized. After being transfected by the transfection complexes, the MMP-2 expression of KYSE150 cells, which overexpresses MMP-2, were examined by Western blot analysis and real-time polymerase chain reaction (PCR). Cell migration and invasion were measured with migration assay and Boyden chamber assays, respectively.
RNAi against MMP-2 successfully inhibited the mRNA and protein expression of MMP-2 in the esophageal carcinoma cell line KYSE150. MMP-2 knockdown inhibited the invasion and migration of esophageal carcinoma cell line KYSE150.
These findings suggested that the RNAi approach towards MMP-2 may be a potentially effective therapeutic method for the treatment of esophageal carcinoma.
食管癌是中国主要的恶性肿瘤之一。既往研究表明，基质金属蛋白酶（matrix metalloproteinases，MMPs）在多种实体瘤的肿瘤侵袭和转移过程中发挥重要作用。在所有 MMPs 中，MMP-2 与肿瘤侵袭关系最为密切。本研究采用 RNA 干扰技术抑制 MMP-2 表达，观察其对高表达 MMP-2 的食管癌 KYSE150 细胞侵袭和迁移的抑制作用。
设计并合成针对 MMP-2mRNA 的 3 个靶向序列的 siRNA，转染高表达 MMP-2 的 KYSE150 细胞后，通过 Western blot 分析和实时聚合酶链反应（polymerase chain reaction，PCR）检测 MMP-2 的表达，采用细胞迁移实验和 Boyden 小室侵袭实验检测细胞的迁移和侵袭能力。
RNAi 可有效抑制食管癌 KYSE150 细胞中 MMP-2mRNA 和蛋白的表达，MMP-2 表达下调可抑制食管癌 KYSE150 细胞的侵袭和迁移。
RNAi 抑制 MMP-2 表达可能成为食管癌治疗的一种潜在有效方法。
