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亲水作用液相色谱（HILIC）——一种强大的分离技术。

Hydrophilic interaction liquid chromatography (HILIC)--a powerful separation technique.

机构信息

Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Toruń, Poland.

出版信息

Anal Bioanal Chem. 2012 Jan;402(1):231-47. doi: 10.1007/s00216-011-5308-5. Epub 2011 Aug 31.

DOI:10.1007/s00216-011-5308-5
PMID:21879300
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3249561/
Abstract

Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to effectively separate small polar compounds on polar stationary phases. The purpose of this work was to review the options for the characterization of HILIC stationary phases and their applications for separations of polar compounds in complex matrices. The characteristics of the hydrophilic stationary phase may affect and in some cases limit the choices of mobile phase composition, ion strength or buffer pH value available, since mechanisms other than hydrophilic partitioning could potentially occur. Enhancing our understanding of retention behavior in HILIC increases the scope of possible applications of liquid chromatography. One interesting option may also be to use HILIC in orthogonal and/or two-dimensional separations. Bioapplications of HILIC systems are also presented.

摘要

亲水作用液相色谱（HILIC）为有效分离极性固定相上的小极性化合物提供了一种替代方法。本工作旨在综述 HILIC 固定相的特性及其在复杂基质中极性化合物分离中的应用。亲水固定相的特性可能会影响并在某些情况下限制可选择的流动相组成、离子强度或缓冲 pH 值，因为可能会发生除亲水分配以外的其他机制。增加对 HILIC 保留行为的理解可扩大液相色谱的应用范围。另一个有趣的选择可能是在正交和/或二维分离中使用 HILIC。还介绍了 HILIC 系统的生物应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/5d7927f11b63/216_2011_5308_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/c6a80f32d2e9/216_2011_5308_Figa_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/59e0903c04e3/216_2011_5308_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/f97a2c728fbe/216_2011_5308_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/88d3c747c432/216_2011_5308_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/74e491f73c15/216_2011_5308_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/ef3308059551/216_2011_5308_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/74fd1427c738/216_2011_5308_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/5c9941a36578/216_2011_5308_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/8dd0c7a07d70/216_2011_5308_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/b4613ebdd59d/216_2011_5308_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/2313f85ac75e/216_2011_5308_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/81f363053d7e/216_2011_5308_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/5d7927f11b63/216_2011_5308_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/c6a80f32d2e9/216_2011_5308_Figa_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/59e0903c04e3/216_2011_5308_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/f97a2c728fbe/216_2011_5308_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/88d3c747c432/216_2011_5308_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/74e491f73c15/216_2011_5308_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/ef3308059551/216_2011_5308_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/74fd1427c738/216_2011_5308_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/5c9941a36578/216_2011_5308_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/8dd0c7a07d70/216_2011_5308_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/b4613ebdd59d/216_2011_5308_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/2313f85ac75e/216_2011_5308_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/81f363053d7e/216_2011_5308_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c140/3249561/5d7927f11b63/216_2011_5308_Fig12_HTML.jpg

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