Institut Supérieur de Biotechnologie, Technopole Sidi Thabet, Université la Manouba, 2020 Ariana, Tunisie.
Ann Clin Microbiol Antimicrob. 2011 Aug 31;10:32. doi: 10.1186/1476-0711-10-32.
For centuries, plants have been used in traditional medicines and there has been recent interest in the chemopreventive properties of compounds derived from plants. In the present study, we investigated the antibutyrylcholinestrasic (anti-BuChE) and antioxidant (against some free radicals) activities of extracts from Rhus pentaphyllum. Aqueous extracts were prepared from powdered R. pentaphyllum roots, leaves and seeds and characterized for the presence of tannins, flavonoids and coumarins. Seeds aqueous extract contained the highest quantities of both flavonoids and tannins (21.12% and 17.45% respectively). In the same way, seeds extracts displayed remarkable inhibition against BuChE over 95%, at 100 μg/ml and with IC50 0.74 μg/ml. In addition, compared to leaves and roots extracts, seeds aqueous extract revealed relatively strong antiradical activity towards the ABTS.+ (IC50 = 0.25 μg/ml) and DPPH (IC50 = 2.71 μg/ml) free radicals and decreased significantly the reactive oxygen species such O2.- (IC50 = 2.9 μg/ml) formation evaluated by the non-enzymatic generating O2.- system (Nitroblue tetrazolium/riboflavine). These data suggest that the anti-BuChE activities mechanism of these extracts occurs through a free radical scavenging capacities.The present study indicates that extracts of Rhus pentaphyllum leaves, seeds and roots are a significant source of compounds, such as tannins, flavonoids and coumarins, with anti-BuChE and antioxidant activities, and thus may be useful for chemoprevention.
几个世纪以来，植物一直被用于传统药物中，最近人们对植物衍生化合物的化学预防特性产生了兴趣。在本研究中，我们研究了五叶地锦提取物的抗丁酰胆碱酯酶（anti-BuChE）和抗氧化（针对一些自由基）活性。从五叶地锦的粉末根、叶和种子中制备了水提取物，并对其是否存在单宁、类黄酮和香豆素进行了表征。种子水提取物同时含有最高量的类黄酮和单宁（分别为 21.12%和 17.45%）。同样，种子提取物对 BuChE 的抑制作用超过 95%，在 100μg/ml 时 IC50 为 0.74μg/ml。此外，与叶和根提取物相比，种子水提取物对 ABTS.+（IC50=0.25μg/ml）和 DPPH（IC50=2.71μg/ml）自由基具有相对较强的抗自由基活性，并且显著降低了由非酶产生的 O2.-（IC50=2.9μg/ml）的形成。通过硝基四唑蓝/核黄素系统（Nitroblue tetrazolium/riboflavine）评价活性氧（reactive oxygen species，ROS）。这些数据表明，这些提取物的抗 BuChE 活性机制是通过自由基清除能力实现的。本研究表明，五叶地锦的叶、种子和根提取物是一种重要的化合物来源，如单宁、类黄酮和香豆素，具有抗 BuChE 和抗氧化活性，因此可能对化学预防有用。
