Feitosa C M, Freitas R M, Luz N N N, Bezerra M Z B, Trevisan M T S
Pós-graduação em Ciências Farmacêuticas, UFPI, Teresina, PI, Brazil.
Braz J Biol. 2011 Aug;71(3):783-9. doi: 10.1590/s1519-69842011000400025.
A microplate assay and a thin-layer chromatography (TLC) "in situ" assay based on the Ellman assay was used to screen for acetylcholinesterase inhibitors from ethyl acetate and methanol extracts of Brazilian medicinal plants of families that, according to the literature, have traditional uses that might be connected with acetylcholinesterase inhibition. Eighteen species belonging to Convolvulaceae, Crassulaceae, Euphorbiaceae, Leguminosae, Malvaceae, Moraceae, Nyctaginaceae and Rutaceae families were tested. The most active plants were Ipomoea asarifolia (IC50 = 0.12 mg/mL), Jatropha curcas (IC50 = 0.25 mg/mL), Jatropha gossypiifolia (IC50 = 0.05 mg/mL), Kalanchoe brasiliensis (IC50 = 0.16 mg/mL) and Senna alata (IC50 = 0.08 mg/mL). The most promising extracts were the Jatropha gossypiifolia and Senna alata species assuming there were compounds with a similar activity to galanthamine, which should contain about 1% of an active compound, or if present at lower levels even more active compounds than galanthamine (IC50 = 0.37 x 10-3 mg/mL) should be present.
基于埃尔曼检测法，采用微孔板检测法和薄层色谱（TLC）“原位”检测法，从巴西药用植物的乙酸乙酯提取物和甲醇提取物中筛选乙酰胆碱酯酶抑制剂。这些植物来自旋花科、景天科、大戟科、豆科、锦葵科、桑科、紫茉莉科和芸香科，据文献记载，它们具有可能与抑制乙酰胆碱酯酶相关的传统用途。对隶属于这些科的18个物种进行了测试。活性最强的植物有细辛叶番薯（IC50 = 0.12毫克/毫升）、麻风树（IC50 = 0.25毫克/毫升）、棉叶麻风树（IC50 = 0.05毫克/毫升）、巴西落地生根（IC50 = 0.16毫克/毫升）和翅荚决明（IC50 = 0.08毫克/毫升）。假设存在与加兰他敏活性相似的化合物，最有前景的提取物是棉叶麻风树和翅荚决明物种，这些化合物应含有约1%的活性化合物，或者即使含量较低，也应存在比加兰他敏（IC50 = 0.37×10-3毫克/毫升）活性更高的化合物。
