Jee Hyunseok, Park Eunmi, Hur Kyunghoon, Kang Minjeong, Kim Yoosik
School of Kinesiology, Yeungnam University, Gyeongsan, South Korea.
College of Life Science and Nano Technology, Hannam University, Daejeon, South Korea.
Front Mol Biosci. 2022 Jun 21;9:818470. doi: 10.3389/fmolb.2022.818470. eCollection 2022.
High-intensity aerobic exercise (90% of the maximal heart rate) can effectively suppress cancer cell proliferation . However, the molecular effects of exercise and its relevance to cancer prevention remain uninvestigated. In this study, mice with colorectal cancer were subjected to high-intensity aerobic exercise, and mRNA-seq analysis was performed on the heart, lungs, and skeletal muscle tissues to analyze the genome-wide molecular effects of exercise. The skeletal muscle-derived genes with exercise-dependent differential expression were further evaluated for their effects on colorectal cancer cell viability. Compared to the results obtained for the control groups (healthy and cancer with no exercise), the regular and high-intensity aerobic physical activity in the mice produced positive results in comprehensive parameters (i.e., food intake, weight gain, and survival rate). A heatmap of differentially expressed genes revealed markedly different gene expression patterns among the groups. RNA-seq analysis of 23,282 genes expressed in the skeletal muscle yielded several anticancer effector genes (e.g., , , , and ). Knockdown and overexpression of selected anticancer genes repressed CT26 murine colorectal carcinoma cell proliferation by 20% ( < 0.05). Our findings, based on the aerobic exercise cancer mouse model, suggest that high-intensity aerobic exercise results in a comprehensive change in the expression patterns of genes, particularly those that can affect cancer cell viability. Such an approach may identify key exercise-regulated genes that can help the body combat cancer.
高强度有氧运动（最大心率的90%）可有效抑制癌细胞增殖。然而，运动的分子效应及其与癌症预防的相关性仍未得到研究。在本研究中，对患有结直肠癌的小鼠进行高强度有氧运动，并对心脏、肺和骨骼肌组织进行mRNA测序分析，以分析运动对全基因组的分子效应。进一步评估骨骼肌来源的、具有运动依赖性差异表达的基因对结直肠癌细胞活力的影响。与对照组（健康且未运动的癌症组）的结果相比，小鼠的规律和高强度有氧体育活动在综合参数（即食物摄入量、体重增加和存活率）方面产生了积极结果。差异表达基因的热图显示各组之间基因表达模式明显不同。对骨骼肌中表达的23282个基因进行RNA测序分析，得到了几个抗癌效应基因（如 、 、 和 ）。敲低和过表达选定的抗癌基因可使CT26小鼠结直肠癌细胞增殖抑制20%（ < 0.05）。基于有氧运动癌症小鼠模型的我们的研究结果表明，高强度有氧运动导致基因表达模式发生全面变化，特别是那些可影响癌细胞活力的基因。这种方法可能会识别出有助于身体对抗癌症的关键运动调节基因。
