College of Life Science, Nantong University, 9 Seyuan Rd, Nantong 226019, People's Republic of China.
J Ind Microbiol Biotechnol. 2012 Feb;39(2):299-305. doi: 10.1007/s10295-011-1031-7. Epub 2011 Sep 1.
The biotransformation of puerarin catalyzed by Bacillus cereus NT02 was studied. A primary screening was carried out using 307 strains of bacteria isolated from soil which were able to grow in the presence of puerarin. Strain NT02, identified as B. cereus, was able to convert puerarin into puerarin-6″-O-phosphate. Under the optimum conditions, resting cells of B. cereus NT02 converted 27% of added 0.4 g/l puerarin into puerarin-6″-O-phosphate that was characterized by MS, ¹³C NMR, ³¹P NMR. The activity of puerarin-6″-O-phosphate was 25 times higher than that of puerarin in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging system. The water solubility of puerarin-6″-O-phosphate was 85.4 times higher than that of puerarin.
研究了蜡状芽孢杆菌 NT02 催化的葛根素的生物转化。通过对 307 株能在葛根素存在下生长的土壤分离菌进行初步筛选，鉴定出能将葛根素转化为葛根素-6″-O-磷酸的菌株 NT02 为蜡状芽孢杆菌。在最佳条件下，蜡状芽孢杆菌 NT02 的静止细胞将 0.4 g/l 加入的葛根素的 27%转化为葛根素-6″-O-磷酸，其特征通过 MS、¹³C NMR、³¹P NMR 进行鉴定。在 1,1-二苯基-2-苦基肼（DPPH）自由基清除系统中，葛根素-6″-O-磷酸的活性比葛根素高 25 倍。葛根素-6″-O-磷酸的水溶性比葛根素高 85.4 倍。
