Key Laboratory for Molecular Animal Nutrition of Ministry of Education, Feed Science Institute, Zhejiang University, Hangzhou 310029, People's Republic of China.
Biol Trace Elem Res. 2012 Mar;145(3):355-60. doi: 10.1007/s12011-011-9191-x. Epub 2011 Sep 1.
The experiment was conducted to evaluate the effect of copper-loaded chitosan nanoparticles on the small intestinal morphology and activities of digestive enzyme and mucosal disaccharase in rats. Forty male Sprague-Dawley rats, with average body weight of 82 g, were randomly allotted to five groups (n = 8). All rats were received a basal diet (control) or the same basal diet added with 80 mg/kg BW CuSO(4), 80 mg/kg BW chitosan (CS-I), 80 mg/kg BW copper-loaded chitosan nanoparticles (CSN-I), 160 mg/kg BW copper-loaded chitosan nanoparticles (CSN-II), respectively. The experiment lasted 21 days. The results showed that the villus heights of the small intestinal mucosa in groups CSN-I and CSN-II were higher than those of the control, group CuSO(4) or CS-I. The crypt depth of duodenum and ileum mucosa in group CSN-I or CSN-II was depressed. Compared with the control, there were no significant effects of CuSO(4) or CS-I on the villus height and crypt depth of small intestinal mucosa. Supplementation with CSN improved the activities of trypsin, amylase and lipase in the small intestinal contents and maltase, sucrase and lactase of duodenum, jejunum, and ileum mucosa while there were no significant effects of CuSO(4) on the digestive enzyme activities of the small content compared with the control. The results indicated that intestinal morphology, activities of digestive enzyme in digesta and mucosal disaccharase were beneficially changed by treatment of copper-loaded chitosan nanoparticles.
该实验旨在评估载铜壳聚糖纳米粒子对大鼠小肠形态和消化酶及黏膜二糖酶活性的影响。将 40 只雄性 Sprague-Dawley 大鼠，平均体重 82g，随机分为 5 组（n=8）。所有大鼠均给予基础日粮（对照组）或相同基础日粮添加 80mg/kg BW CuSO4、80mg/kg BW 壳聚糖（CS-I）、80mg/kg BW 载铜壳聚糖纳米粒子（CSN-I）、160mg/kg BW 载铜壳聚糖纳米粒子（CSN-II）。实验持续 21 天。结果表明，CSN-I 和 CSN-II 组的小肠黏膜绒毛高度高于对照组、CuSO4 组和 CS-I 组。CSN-I 和 CSN-II 组的十二指肠和回肠黏膜隐窝深度降低。与对照组相比，CuSO4 或 CS-I 对小肠黏膜绒毛高度和隐窝深度无显著影响。CSN 的补充提高了小肠内容物中胰蛋白酶、淀粉酶和脂肪酶以及十二指肠、空肠和回肠黏膜中麦芽糖酶、蔗糖酶和乳糖酶的活性，而 CuSO4 对小肠内容物中消化酶的活性与对照组相比无显著影响。结果表明，载铜壳聚糖纳米粒子处理可有益地改变肠道形态、消化酶活性和黏膜二糖酶。
