A total of 320 1-d-old Arbor Acres broiler chicks were used to investigate the effect of Cu(2+)-loaded montmorillonite (CM) on the growth performance, intestinal morphology and activities of brush border enzyme in the intestinal mucosa and digestive enzyme in the intestinal digesta of broilers. 2. The chicks were assigned randomly into 4 groups with 80 chicks per treatment. The 4 dietary treatments were: basal diet only (control group), basal diet + 2 g montmorillonite/kg, basal diet + 1 g CM/kg, and basal diet + 2 g CM/kg. The chicks were raised in cages and feed and water were provided ad libitum for a period of 42 d. 3. The addition of CM to the diet of broilers significantly increased body weight and feed efficiency. Similarly, birds receiving montmorillonite had higher feed efficiency than the control after 42 d of feeding. 4. Data on villus height and crypt depth for duodenum, jejunum and ileum indicated that treating the diet of broilers with either CM or montmorillonite improved the mucosal morphology of the small intestine. 5. The presence of CM in the diet of broilers significantly increased the activities of maltase, aminopeptidase N and alkaline phosphatase in small intestinal mucosa. However, the activities of protease, trypsin, chymotrypsin, amylase and lipase in small intestinal digesta of broilers fed on the CM-supplemented diet were slightly higher than control values.
摘要
总共320只1日龄的艾维茵肉鸡雏鸡被用于研究载铜蒙脱石（CM）对肉鸡生长性能、肠道形态、肠黏膜刷状缘酶活性以及肠内容物消化酶活性的影响。2. 雏鸡被随机分为4组，每组80只。4种日粮处理分别为：仅基础日粮（对照组）、基础日粮+2 g蒙脱石/kg、基础日粮+1 g CM/kg以及基础日粮+2 g CM/kg。雏鸡饲养在笼中，自由采食和饮水，为期42天。3. 在肉鸡日粮中添加CM显著增加了体重和饲料效率。同样，饲喂42天后，采食蒙脱石的鸡比对照组具有更高的饲料效率。4. 十二指肠、空肠和回肠绒毛高度和隐窝深度的数据表明，用CM或蒙脱石处理肉鸡日粮可改善小肠黏膜形态。5. 在肉鸡日粮中添加CM显著提高了小肠黏膜中麦芽糖酶、氨肽酶N和碱性磷酸酶的活性。然而，采食添加CM日粮的肉鸡小肠内容物中蛋白酶、胰蛋白酶、糜蛋白酶、淀粉酶和脂肪酶的活性略高于对照值。
