Chemistry Department, Faculty of Science, Suez Canal University, Ismailia, 41522, Egypt.
J Fluoresc. 2012 Jan;22(1):223-38. doi: 10.1007/s10895-011-0949-5. Epub 2011 Sep 2.
Two new ligands derived from phloroglucinol 2-{[(4-methoxy benzoyl)oxy]} methyl benzoic acid[L1] and 2-{[(4-methyl benzoyl)oxy] methyl} benzoic acid[L2] were synthesized. The solid complex Eu(III)-L2 has been synthesised and characterized by elemental analysis, UV and IR spectra. The reaction of Eu(III) with the two synthesized ligands has been investigated in I = 0.1 mol dm(-3) p-toluene sulfonate by cyclic voltammetry and square wave voltammetry. The reaction of Eu (III)-L1 and Eu (III)-L2 binary complexes with nucleotide 5'-AMP, 5'-ADP, 5'-ATP, 5'- GMP, 5'-IMP, and 5'-CMP has been investigated using UV, fluorescence and electrochemical methods. The experimental conditions were selected such that self-association of the nucleotides and their complexes was negligibly small, that is, the monomeric complexes were studied. The interaction of the Eu(III)-L1 or L2 solid complexes with calf-thymus DNA has been investigated by fluorescence and electrochemical methods including cyclic voltammetery(CV), differential pulse polarography (DPP) and square wave voltammetry (SWV) on a glassy carbon electrode. The fluorescence intensity of Eu(III)-L2 complex was enhanced with the addition of DNA. Under optimal conditions in phosphate buffer pH 7.0 at 25 °C the linear range is 3-20 μM for calf thymus DNA (CT-DNA) and the corresponding determination limit is 1.8 μM.
合成了两种新的间苯三酚配体 2-[(4-甲氧基苯甲酰基)氧基]甲基苯甲酸[L1]和 2-[(4-甲基苯甲酰基)氧基]甲基苯甲酸[L2]。通过元素分析、紫外和红外光谱对 Eu(III)-L2 固体配合物进行了合成和表征。通过循环伏安法和方波伏安法研究了 Eu(III)与两种合成配体的反应。使用紫外、荧光和电化学方法研究了 Eu (III)-L1 和 Eu (III)-L2 二元配合物与核苷酸 5'-AMP、5'-ADP、5'-ATP、5'-GMP、5'-IMP 和 5'-CMP 的反应。选择的实验条件使得核苷酸及其配合物的自缔合可以忽略不计，即研究了单体配合物。通过荧光和电化学方法（包括循环伏安法、微分脉冲极谱法和方波伏安法）在玻碳电极上研究了 Eu(III)-L1 或 L2 固体配合物与小牛胸腺 DNA 的相互作用。Eu(III)-L2 配合物的荧光强度随着 DNA 的加入而增强。在 25°C 磷酸盐缓冲液 pH 7.0 的最佳条件下，小牛胸腺 DNA（CT-DNA）的线性范围为 3-20 μM，相应的检测限为 1.8 μM。
