Department of Biochemistry and Molecular Pathology, Osaka City University Medical School, Osaka, Japan.
Clin Exp Dermatol. 2012 Jan;37(1):65-7. doi: 10.1111/j.1365-2230.2011.04149.x. Epub 2011 Aug 25.
Irradiation by ultraviolet (UV)B is known to increase the number of dopamine (Dopa)-positive melanocytes in the skin. In this study, a 2.5-kJ/m(2) dose of UVB radiation was delivered by a sunlamp to the ear or the eye of wild-type C57BL/6j mice and of gp91 phox(-/-) C57BL/6j mice that had a knockout mutation of the gp91 phox subunit of reduced nicotinamide adenine dinucleotide phosphate oxidase (NADPH). The degree of change in the Dopa-positive melanocyte expression in was reduced in gp91 phox(-/-) mice given UVB irradiation to the eye, but not in those given irradiation to the ear. The plasma level of α-melanocyte-stimulating hormone (α-MSH) in the blood increased in the C57BL/6j mice after irradiation to either the eye or the ear, but it did not increase in the gp91 phox(-/-) mice given UVB irradiation to the eye. Both gp91 phox and α-MSH in the central nervous system seem to contribute to pigmentation after UVB irradiation of the eye in mice.
已知紫外线（UVB）照射可增加皮肤中多巴胺（Dopa）阳性黑素细胞的数量。在这项研究中，通过太阳灯向野生型 C57BL/6j 小鼠和烟酰胺腺嘌呤二核苷酸磷酸氧化酶（NADPH）还原型 gp91 phox 亚单位缺失突变的 gp91 phox(-/-) C57BL/6j 小鼠的耳朵或眼睛照射 2.5kJ/m(2)剂量的 UVB 辐射。与接受耳部 UVB 照射的 gp91 phox(-/-) 小鼠相比，眼部接受 UVB 照射的 gp91 phox(-/-) 小鼠的 Dopa 阳性黑素细胞表达变化程度降低。在接受眼部或耳部照射后，C57BL/6j 小鼠的血液中α-促黑素细胞激素（α-MSH）的血浆水平升高，但眼部接受 UVB 照射的 gp91 phox(-/-) 小鼠的α-MSH 水平没有升高。gp91 phox 和中枢神经系统中的 α-MSH 似乎都有助于小鼠眼部接受 UVB 照射后的色素沉着。
