Department of Fish Nutrition and Biochemistry, Central Institute of Fisheries Education, Versova, Mumbai, India.
J Anim Physiol Anim Nutr (Berl). 2012 Dec;96(6):1012-9. doi: 10.1111/j.1439-0396.2011.01215.x. Epub 2011 Aug 29.
A feeding trial was conducted for 60 days to delineate the effect of alternate day feeding strategy of sub-optimal protein level on haematological parameters, serum parameters and phagocyte respiratory burst activity (NBT) in Labeo rohita juveniles. One hundred and thirty-five fingerlings (1.87 ± 0.01-2.26 ± 0.05 g) were distributed in triplicate groups of each treatment, and fish were fed at 5% body weight daily. Three experimental isocaloric (401.32-410.28 kcal/100 g) diets of 30%, 25%, and 20% crude protein designated as diet A, diet B, and diet C respectively, were prepared, using locally available feed ingredients. Three different feeding schedules of normal protein diet continuously (diet A-30%), alternate feeding of 1-day diet A followed by 1-day diet B (1A/1B) and alternate feeding of 1-day diet A followed by 1-day diet C (1A/1C) were tested. The total erythrocyte count and haemoglobin content was significantly (p < 0.05) enhanced in the group T1 fed (1A/1B), and the lowest count was recorded in the group T2 fed (1A/1C). Total leucocyte counts, total serum protein, and serum globulin were higher in the group T1 fed (1A/1B) and lower in the group T2 fed (1A/1C) as compare to control. The respiratory burst activity (NBT) of blood phagocytes and serum A-to-G ratio was recorded significantly difference in among the treatment groups. Based on the results of the present study, it is concluded that alternate feeding of 1-day diet A followed by 1-day diet B (1A/1B) is equally effective and promote the immunity in Labeo rohita juveniles.
进行了为期 60 天的饲养试验，以描绘亚最佳蛋白质水平的隔日喂养策略对罗非鱼幼鱼血液学参数、血清参数和吞噬细胞呼吸爆发活性（NBT）的影响。将 135 尾（1.87±0.01-2.26±0.05g）幼鱼分配到每个处理的三重复组中，每天按体重的 5%投喂。使用当地可用的饲料原料，制备了三种等热量（401.32-410.28 千卡/100 克）的实验饲料，分别命名为饲料 A、饲料 B 和饲料 C，粗蛋白含量分别为 30%、25%和 20%。连续投喂正常蛋白饲料（饲料 A-30%）、隔日投喂 1 天饲料 A 后 1 天饲料 B（1A/1B）和隔日投喂 1 天饲料 A 后 1 天饲料 C（1A/1C）三种不同的喂养方案进行了测试。在 T1 组（1A/1B）投喂的情况下，总红细胞计数和血红蛋白含量显著增加（p<0.05），而在 T2 组（1A/1C）投喂的情况下，总红细胞计数最低。T1 组（1A/1B）投喂的总白细胞计数、总血清蛋白和血清球蛋白较高，而 T2 组（1A/1C）投喂的总白细胞计数、总血清蛋白和血清球蛋白较低。与对照组相比，血液吞噬细胞的呼吸爆发活性（NBT）和血清 A-G 比值在各组之间存在显著差异。根据本研究的结果，结论是隔日投喂 1 天饲料 A 后 1 天饲料 B（1A/1B）同样有效，并能促进罗非鱼幼鱼的免疫力。
