Minnet Coskun, Koc Ahmet, Aycicek Ali, Kocyigit Abdurrahim
Department of Pediatrics, Eskisehir Sakarya Hospital, Eskisehir, Turkey.
Pediatr Int. 2011 Dec;53(6):1023-7. doi: 10.1111/j.1442-200X.2011.03448.x. Epub 2011 Oct 30.
DNA damage effects of vitamin B(12) deficiency were performed in vitro and in adults.
The study group included 32 children (13 girls, 19 boys) with vitamin B(12) deficiency (mean age 44 ± 58 months) and their 27 mothers (mean age 30.4 ± 5.3 years). The control group contained 30 healthy children and 25 mothers. DNA strand breaks in peripheral blood mononuclear leukocytes were assayed by single-cell alkaline gel electrophoresis (comet assay) before and 8 days after the first injection of vitamin B(12).
Mean DNA damage scores in children with vitamin B(12) deficiency and their mothers were significantly higher before treatment than those after treatment. The DNA damage scores of children after treatment were still significantly higher than controls. There were significant negative correlations between the children and their mothers in terms of vitamin B(12) levels and DNA damage scores (r = 0.3, P= 0.02; r = 0.58, P= 0.002, respectively). There were correlations between the children's and their mothers' DNA damage and the severity of vitamin B(12) deficiency, suggesting that the children and their mothers may play a role in the scarcity of nutritional vitamin B(12).
DNA damage is increased in children with vitamin B(12) deficiency and in their mothers. DNA damage scores were significantly improved through vitamin B(12) therapy 8 days after the first injection, however, they were still significantly higher than those of controls.
在体外和成人中进行了维生素B12缺乏的DNA损伤效应研究。
研究组包括32名维生素B12缺乏的儿童（13名女孩，19名男孩）（平均年龄44±58个月）及其27名母亲（平均年龄30.4±5.3岁）。对照组包含30名健康儿童和25名母亲。在首次注射维生素B12之前和之后8天，通过单细胞碱性凝胶电泳（彗星试验）检测外周血单核白细胞中的DNA链断裂情况。
维生素B12缺乏儿童及其母亲治疗前的平均DNA损伤评分显著高于治疗后。治疗后儿童的DNA损伤评分仍显著高于对照组。儿童及其母亲在维生素B12水平和DNA损伤评分方面存在显著负相关（r分别为0.3，P = 0.02；r为0.58，P = 0.002）。儿童及其母亲的DNA损伤与维生素B12缺乏的严重程度之间存在相关性，表明儿童及其母亲可能在营养性维生素B12缺乏中起作用。
维生素B12缺乏的儿童及其母亲的DNA损伤增加。首次注射维生素B12 8天后，通过维生素B12治疗，DNA损伤评分显著改善，然而，仍显著高于对照组。
