Center for Biofilm Engineering, Montana State University, Bozeman, MT, USA.
J Appl Microbiol. 2011 Nov;111(5):1275-82. doi: 10.1111/j.1365-2672.2011.05138.x. Epub 2011 Sep 19.
To develop an in vitro model (Colony/drip-flow reactor - C/DFR) for the growth and analysis of methicillin-resistant Staphylococcus aureus (MRSA) biofilms.
Using the C/DFR model, biofilms were grown on the top of polycarbonate filter membranes inoculated with a clinical isolate of MRSA, placed on absorbent pads in the DFR and harvested after 72 h. The biofilms varied from 256 to 308 μm in thickness with a repeatability standard deviation of 0·22. Testing of antimicrobial agents was also performed where C/DFR biofilms were grown in parallel with conventional colony biofilms. A saline solution (control), 1% silver sulfadiazine solution, and 0·25% Dakin's solution were used to treat the biofilms for 15 min. Microscopic evaluation of biofilm morphology and thickness was conducted. The Dakins solution in both models produced statistically significantly higher log reductions than silver sulfadiazine treatment.
The C/DFR biofilms were thick and repeatable and exhibited higher resistance to Dakins solution than the treated colony biofilms.
The C/DFR can be used as a tool for examining complex biofilm physiology as well as for performing comparative experiments that test wound care products and novel antimicrobials.
开发一种用于生长和分析耐甲氧西林金黄色葡萄球菌（MRSA）生物膜的体外模型（集落/滴流反应器-C/DFR）。
使用 C/DFR 模型，在接种有临床分离的 MRSA 的聚碳酸酯滤膜顶部生长生物膜，将其放置在 DFR 的吸收垫上，72 小时后收获。生物膜厚度在 256 至 308μm 之间，重复性标准偏差为 0.22。还进行了抗菌剂测试，其中 C/DFR 生物膜与常规菌落生物膜平行生长。用生理盐水（对照）、1%磺胺嘧啶银溶液和 0.25%Dakin 溶液处理生物膜 15 分钟。对生物膜形态和厚度进行了显微镜评估。两种模型中的 Dakin 溶液产生的对数减少均明显高于磺胺嘧啶银处理。
C/DFR 生物膜较厚且具有重复性，对 Dakins 溶液的抵抗力高于经处理的菌落生物膜。
C/DFR 可作为一种工具，用于研究复杂的生物膜生理学，并进行比较实验，以测试伤口护理产品和新型抗菌剂。
