D'Atanasio Noemi, Capezzone de Joannon Alessandra, Mangano Giorgina, Meloni Marisa, Giarratana Nadia, Milanese Claudio, Tongiani Serena
Angelini Research S.p.A.,Santa Polumba, Rome, Italy.
VitroScreen Srl, Milan, Italy.
Wounds. 2015 Oct;27(10):265-73.
Biofilms represent a key challenge in the treatment of chronic wounds, as they are among the main reasons for delays in chronic wound healing. This in vitro study was aimed at evaluating the activity of a new acid-oxidizing solution (AOS) on biofilm formation. Acid-oxidizing solution contains free chlorine species with stabilized hypochlorous acid in high concentration (> 95%) and is characterized by acidic (pH less than 3) and super-oxidizing (Redox greater than 1000mV) features.
A 3-dimensional in vitro model of reconstructed human epidermis was used to compare the activity of AOS vs 2 reference products (RP) containing betaine and polyhexanide (RP1) and sodium hypochlorite and hypochlorous acid (RP2). Different approaches were used to assess the prevention and eradication of methicillin-resistant Staphyloccocus aureus biofilm by the study products. Xylitol and chlorhexidine were used as positive controls. The activity of the study products on the biofilm structure was evaluated analyzing the ultrastructural modification by scanning electron microscopy, while skin compatibility was assessed on noncolonized tissues measuring the metabolic activity of the cells.
In all experiments, AOS showed to be active on the biofilm matrix, modifying its structure and allowing bacterial release from the matrix. In all experiments, no cytotoxicity was observed in the tissues treated with the product suggesting a good compatibility of AOS with skin tissues. Reference product 1 affected the biofilm, suggesting a disruption effect; RP2 was slightly less active than AOS in modifying the biofilm structure.
Treatment with AOS affects biofilm by modifying its structure and therefore facilitating local bacteria accessibility to bactericidal agents, with consequent potential clinical benefits in the treatment of chronic wounds.
生物膜是慢性伤口治疗中的一个关键挑战，因为它们是慢性伤口愈合延迟的主要原因之一。这项体外研究旨在评估一种新型酸氧化溶液（AOS）对生物膜形成的活性。酸氧化溶液含有高浓度（>95%）的稳定次氯酸的游离氯物质，其特点是呈酸性（pH值小于3）和具有超强氧化性（氧化还原电位大于1000mV）。
使用重建人表皮的三维体外模型来比较AOS与两种含甜菜碱和聚己双胍的参考产品（RP1）以及含次氯酸钠和次氯酸的参考产品（RP2）的活性。采用不同方法评估研究产品对耐甲氧西林金黄色葡萄球菌生物膜的预防和根除作用。木糖醇和氯己定用作阳性对照。通过扫描电子显微镜分析超微结构变化来评估研究产品对生物膜结构的活性，同时在未定植组织上通过测量细胞代谢活性来评估皮肤相容性。
在所有实验中，AOS对生物膜基质具有活性，改变其结构并使细菌从基质中释放出来。在所有实验中，在用该产品处理的组织中未观察到细胞毒性，这表明AOS与皮肤组织具有良好的相容性。参考产品1对生物膜有影响，显示出破坏作用；RP2在改变生物膜结构方面的活性略低于AOS。
AOS治疗通过改变生物膜结构来影响生物膜，从而促进局部细菌与杀菌剂的接触，因此在慢性伤口治疗中可能具有临床益处。
