Baran Ewelina, Górska Anna, Birczyński Artur, Hudy Wiktor, Kulinowski Wojciech, Jamróz Witold, Węglarz Władysław P, Kulinowski Piotr
Institute of Technology, The Pedagogical University of Kraków, Podchorążych 2, 30-084 Kraków, Poland.
Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna Street, 30-688 Kraków, Poland.
Materials (Basel). 2021 Dec 13;14(24):7702. doi: 10.3390/ma14247702.
Wound dressings when applied are in contact with wound exudates in vivo or with acceptor fluid when testing drug release from wound dressing in vitro. Therefore, the assessment of bidirectional mass transport phenomena in dressing after application on the substrate is important but has never been addressed in this context. For this reason, an in vitro wound dressing stack model was developed and implemented in the 3D printed holder. The stack was imaged using magnetic resonance imaging, i.e., relaxometric imaging was performed by means of relaxation time and signal amplitude 1D profiles across the wound stack. As a substrate, fetal bovine serum or propylene glycol were used to simulate in vivo or in vitro cases. Multi-exponential analysis of the spatially resolved magnetic resonance signal enabled to distinguish components originating from water and propylene glycol in various environments. The spatiotemporal evolution of these components was assessed. The components were related to mass transport (water, propylene glycol) in the dressing/substrate system and subsequent changes of physicochemical properties of the dressing and adjacent substrate. Sharp changes in spatial profiles were detected and identified as moving fronts. It can be concluded that: (1) An attempt to assess mass transport phenomena was carried out revealing the spatial structure of the wound dressing in terms of moving fronts and corresponding layers; (2) Moving fronts, layers and their temporal evolution originated from bidirectional mass transport between wound dressing and substrate. The setup can be further applied to dressings containing drugs.
伤口敷料在体内应用时与伤口渗出液接触，或在体外测试伤口敷料的药物释放时与接受液接触。因此，评估敷料在应用于底物后双向传质现象很重要，但在这方面从未有过相关研究。基于此，开发了一种体外伤口敷料堆叠模型，并在3D打印支架中实现。使用磁共振成像对堆叠进行成像，即通过伤口堆叠的弛豫时间和信号幅度一维轮廓进行弛豫测量成像。使用胎牛血清或丙二醇作为底物来模拟体内或体外情况。对空间分辨磁共振信号进行多指数分析，能够区分来自不同环境中与水和丙二醇相关的成分。评估了这些成分的时空演变。这些成分与敷料/底物系统中的传质（水、丙二醇）以及敷料和相邻底物的物理化学性质的后续变化有关。检测到空间轮廓的急剧变化并将其识别为移动前沿。可以得出以下结论：（1）尝试评估传质现象，揭示了伤口敷料在移动前沿和相应层方面的空间结构；（2）移动前沿、层及其时间演变源于伤口敷料和底物之间的双向传质。该装置可进一步应用于含药敷料。
