水凝胶纳米粒子与蛋白质组学的联合应用揭示与人类子宫内膜基质细胞蜕膜化相关的分泌蛋白。
Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells.
机构信息
Prince Henrys Institute of Medical Research, Clayton 3168 Australia.
出版信息
Proteome Sci. 2011 Sep 1;9(1):50. doi: 10.1186/1477-5956-9-50.
Abstract
BACKGROUND
Identification of secreted proteins of low abundance is often limited by abundant and high molecular weight (MW) proteins. We have optimised a procedure to overcome this limitation.
RESULTS
Low MW proteins in the conditioned media of cultured cells were first captured using dual-size exclusion/affinity hydrogel nanoparticles and their identities were then revealed by proteomics.
CONCLUSIONS
This technique enables the analysis of secreted proteins of cultured cells low MW and low abundance.
摘要
背景
低丰度分泌蛋白的鉴定通常受到丰富和高分子量（MW）蛋白的限制。我们已经优化了一种克服这一限制的方法。
结果
先用双尺寸排阻/亲和水凝胶纳米粒子捕获培养细胞条件培养基中的低 MW 蛋白，然后通过蛋白质组学揭示其身份。
结论
该技术能够分析培养细胞中低 MW 和低丰度的分泌蛋白。
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