University of Montreal, Maisonneuve-Rosemont Hospital, Research Center, 5415 de l'Assomption, Montreal, Quebec, Canada H1T 2M4.
Biochem Biophys Res Commun. 2011 Sep 23;413(2):248-53. doi: 10.1016/j.bbrc.2011.08.079. Epub 2011 Aug 23.
In Saccharomyces cerevisiae, the immunosuppressor rapamycin engenders the degradation of excessive RNA polymerase II leading to growth arrest but the regulation of this process is not known yet. Here, we show that this mechanism is dependent on the peptidyl prolyl cis/trans isomerase Rrd1. Strikingly this degradation is independent of RNA polymerase II polyubiquitylation and does not require the elongation factor Elc1. Our data reveal that there are at least two alternative pathways to degrade RNA polymerase II that depend on different type of stresses.
在酿酒酵母中，免疫抑制剂雷帕霉素引发过量 RNA 聚合酶 II 的降解，导致生长停滞，但这一过程的调控机制尚不清楚。在这里，我们表明这种机制依赖于肽酰脯氨酰顺/反式异构酶 Rrd1。引人注目的是，这种降解不依赖于 RNA 聚合酶 II 的多泛素化，也不需要延伸因子 Elc1。我们的数据表明，至少有两种替代途径可以降解 RNA 聚合酶 II，它们依赖于不同类型的应激。
