Istanbul University, Cerrahpasa Medical Faculty, Department of Biochemistry, Istanbul, Turkey.
Diabetes Res Clin Pract. 2011 Dec;94(3):404-9. doi: 10.1016/j.diabres.2011.08.001. Epub 2011 Aug 31.
To clarify the levels of protein oxidation markers such as protein carbonyl (PCO), protein hydroperoxides (P-OOH), advanced oxidation protein products (AOPP) and nitrotyrosine (NT), as well as antioxidative enzymes such as paraoxonase (PON-1) in women with and without gestational diabetes mellitus (GDM).
The study was conducted on 23 women with GDM and 22 women without GDM. The levels of the P-OOH, AOPP, and PON-1 were determined by colorimetric methods; whereas NT and PCO levels were measured by ELISA.
The concentrations of protein oxidation markers were significantly increased and PON1 activity was significantly decreased in GDM group compared to those of normal pregnant women. The control group showed a significant negative correlation between PON-1 and PCO (r=-0.451, p=0.027); whereas in GDM group, there was a significant positive correlation between P-OOH and HbA1c (r=0.89, p=0.001). There was no significant correlation between AOPP, PON-1, P-OOH, PCO, and HbA1c in either group.
There is evidence of a possible association between protein oxidation and decreased PON1 activity in GDM. The increase in protein oxidation parameters in the GDM group leading to decreased PON1 activity might, we think, create a predisposition for clinical complications in GDM group.
明确蛋白质氧化标志物（如蛋白羰基（PCO）、蛋白过氧化物（P-OOH）、晚期氧化蛋白产物（AOPP）和硝基酪氨酸（NT））以及抗氧化酶（如对氧磷酶 1（PON-1））在患有和不患有妊娠期糖尿病（GDM）的女性中的水平。
该研究纳入了 23 名患有 GDM 的女性和 22 名不患有 GDM 的女性。通过比色法测定 P-OOH、AOPP 和 PON-1 的水平；通过 ELISA 法测定 NT 和 PCO 的水平。
与正常孕妇相比，GDM 组的蛋白质氧化标志物浓度显著升高，PON1 活性显著降低。对照组中 PON-1 与 PCO 呈显著负相关（r=-0.451，p=0.027）；而在 GDM 组中，P-OOH 与 HbA1c 呈显著正相关（r=0.89，p=0.001）。两组中 AOPP、PON-1、P-OOH、PCO 和 HbA1c 之间均无显著相关性。
有证据表明，GDM 中可能存在蛋白质氧化和 PON1 活性降低之间的关联。GDM 组中蛋白质氧化参数的增加导致 PON1 活性降低，可能使 GDM 组更容易发生临床并发症。
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