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鉴定拟南芥 ABA 信号中 2C 型蛋白磷酸酶 ABI2 功能的重要位点。

Identification of an important site for function of the type 2C protein phosphatase ABI2 in abscisic acid signalling in Arabidopsis.

机构信息

College of Biological Sciences, China Agricultural University, Beijing 100094, China.

出版信息

J Exp Bot. 2011 Nov;62(15):5713-25. doi: 10.1093/jxb/err274. Epub 2011 Sep 1.

DOI:10.1093/jxb/err274
PMID:21885535
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3223061/
Abstract

It is known that the clade A protein phosphatase 2Cs (PP2Cs), including ABI1 and ABI2 and other PP2C members, are key players that function directly downstream of the PYR/PYL/RCAR abscisic acid (ABA) receptors. Here, identification of a crucial site for function of ABI2 protein phosphatase in ABA signalling is reported. It was observed that a calcium-dependent protein kinase (CDPK) phosphorylation site-like motif (CPL) in the ABI2 molecule is required for the interactions of ABI2 with the two members of the ABA receptors PYL5 and PYL9 and with a downstream protein kinase SnRK2.6, and for the catalytic activity of ABI2 in vitro, as well as for the response of ABI2 to the ABA receptors PYL5/PYL9 in relation to the ABA receptor-induced inhibition of the ABI2 phosphatase activity. Further, genetic evidence was provided to demonstrate that this CPL is required for the function of ABI2 to mediate ABA signalling. These data reveal that this CPL is an important site necessary for both the phosphatase activity of ABI2 and the functional interaction between ABI2 and PYL5/9 ABA receptors, providing new information to understand primary events of ABA signal transduction.

摘要

已知 clade A 蛋白磷酸酶 2C（PP2Cs），包括 ABI1 和 ABI2 以及其他 PP2C 成员，是直接作用于脱落酸（ABA）受体 PYR/PYL/RCAR 下游的关键因子。本文报道了 ABI2 蛋白磷酸酶在 ABA 信号转导中功能的一个关键位点的鉴定。研究发现，ABI2 分子中的一个钙依赖蛋白激酶（CDPK）磷酸化位点样模体（CPL）对于 ABI2 与 ABA 受体 PYL5 和 PYL9 的两个成员以及下游蛋白激酶 SnRK2.6 的相互作用、ABI2 在体外的催化活性以及 ABI2 对 ABA 受体 PYL5/PYL9 的反应都是必需的，因为 ABA 受体诱导的 ABI2 磷酸酶活性抑制。此外，还提供了遗传证据来证明这个 CPL 对于 ABI2 介导 ABA 信号转导的功能是必需的。这些数据表明，这个 CPL 是 ABI2 的磷酸酶活性和 ABI2 与 PYL5/9 ABA 受体之间功能相互作用所必需的重要位点，为理解 ABA 信号转导的初始事件提供了新的信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d2d/3223061/12eee5c36e74/jexboterr274f07_3c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d2d/3223061/33aca08ae650/jexboterr274f01_3c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d2d/3223061/874d3bc81a3a/jexboterr274f02_3c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d2d/3223061/69a848024448/jexboterr274f03_ht.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d2d/3223061/c17e033ebf74/jexboterr274f04_lw.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d2d/3223061/963d17dddebe/jexboterr274f05_3c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d2d/3223061/f09513b874b2/jexboterr274f06_3c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d2d/3223061/12eee5c36e74/jexboterr274f07_3c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d2d/3223061/33aca08ae650/jexboterr274f01_3c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d2d/3223061/874d3bc81a3a/jexboterr274f02_3c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d2d/3223061/69a848024448/jexboterr274f03_ht.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d2d/3223061/c17e033ebf74/jexboterr274f04_lw.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d2d/3223061/963d17dddebe/jexboterr274f05_3c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d2d/3223061/f09513b874b2/jexboterr274f06_3c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d2d/3223061/12eee5c36e74/jexboterr274f07_3c.jpg

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