Identification and characterization of playing a positive role in the abiotic stress resistance of Chinese chestnut via an integrated strategy.

Chinese chestnut ( Blume) is an important economic forest tree species and mainly cultivated in mountainous areas and wastelands, subjecting it to various abiotic stresses. The protein phosphatase 2C (PP2C) genes contributes largely to stress responses in plants. However, the characteristics and functions of genes in remain unknown. This study provides comprehensive analyses (including phylogenetic, synteny, RNA-seq, transgenic and yeast one-hybrid methods) revealing the characteristics of gene, which plays an important role in response to abiotic stress. Here, we identified 68 in the Chinese chestnut genome, and analyzed their characteristics and phylogenetic relationships. Furthermore, synteny analysis revealed that segmental and tandem duplication drove the expansion of the family to adapt to natural environmental pressures. RNA sequencing and co-expression analyses indicated that four hub in two key modules probably play important roles in the resistance to abiotic stress in chestnut. Among them, was significantly down-regulated under drought stress. Transgenic experiments via pollen magnetofection revealed that could positively and significantly regulate the drought resistance of Chinese chestnut seedlings. Subcellular localization showed that CmPP2C31 was a nuclear protein. Yeast one-hybrid assays suggested that EVM0007407 could regulate expression by binding to its promoter, thereby participating in abiotic stress resistance. These findings in our study provided detailed information on the family genes and laid a foundation for further elucidating the molecular mechanism of resistance to abiotic stress chestnut.