Castelijns G, Crawford A, Schaffer J, Ortolano G A, Beauregard T, Smith R K W
Equihealth Vetrinarios SL, Real Club de Polo de Barcelona, Barcelona, Spain.
Vet Comp Orthop Traumatol. 2011;24(5):363-9. doi: 10.3415/VCOT-11-01-0001. Epub 2011 Sep 2.
Platelet preparations have become a treatment for soft tissue injuries in horses. This study evaluated a novel filter-based system to concentrate platelets and assesses its value in the treatment of suspensory ligament branch desmitis.
Filtered platelet concentrate was prepared from 55 ml of venous blood obtained from 21 normal horses. Platelets and white blood cells in whole blood and filtered platelet concentrate were measured, as was platelet activating factor (PAF)-induced platelet-derived growth factor-BB (PDGF-BB) release. Eleven horses with 18 focal acute suspensory ligament branch injuries were treated intra-lesionally with autologous filtered platelet concentrate and evaluated clinically and ultrasonographically for one to three years.
The increase in concentration of platelets in the filtered concentrate in comparison with whole blood (6.9 ± 1.9-fold) was significantly greater than the increase in white blood cells (3.8 ± 0.8-fold) (p <0.0001). There was no effect of sex or breed on platelet concentration. Platelets were responsive to PAF with >100-fold higher levels of PDGF release over basal levels. All hypoechoic lesions re-evaluated within three months had resolved. Five of the 11 horses returned to their previous level of work, one was exercising at a lower level, three were retired, one died for unrelated reasons, and one was still convalescing.
Filtered platelet concentrate was easily and reliably prepared and injected into suspensory ligament branch injuries without short-term complications. This treatment was associated with rapid resolution of ultrasonographic lesions and lameness. Filtered platelet concentrate represents a convenient alternative for the treatment of suspensory ligament branch injuries.
血小板制剂已成为治疗马软组织损伤的一种方法。本研究评估了一种基于新型过滤器的系统来浓缩血小板，并评估其在治疗悬韧带分支腱炎中的价值。
从21匹正常马采集55毫升静脉血制备过滤血小板浓缩液。测量全血和过滤血小板浓缩液中的血小板和白细胞，以及血小板活化因子（PAF）诱导的血小板衍生生长因子-BB（PDGF-BB）释放。对11匹患有18处局灶性急性悬韧带分支损伤的马进行病灶内自体过滤血小板浓缩液治疗，并进行一至三年的临床和超声检查评估。
与全血相比，过滤浓缩液中血小板浓度的增加（6.9±1.9倍）显著大于白细胞浓度的增加（3.8±0.8倍）（p<0.0001）。性别和品种对血小板浓度无影响。血小板对PAF有反应，PDGF释放水平比基础水平高100倍以上。在三个月内重新评估的所有低回声病灶均已消退。11匹马中有5匹恢复到以前的工作水平，1匹以较低水平运动，3匹退役，1匹因无关原因死亡，1匹仍在康复中。
过滤血小板浓缩液制备简便、可靠，可注入悬韧带分支损伤部位，无短期并发症。这种治疗与超声病灶和跛行的快速消退有关。过滤血小板浓缩液是治疗悬韧带分支损伤的一种方便的替代方法。
