Bozorgmanesh Rana, Magdesian K Gary, Sutton-Burges Julie W, Owens Sean D, Tablin Fern
McGee Medicine Center, Hagyard Equine Medical Institute, Lexington, Kentucky.
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California.
J Vet Intern Med. 2019 May;33(3):1500-1506. doi: 10.1111/jvim.15472. Epub 2019 Mar 13.
Development of equine platelet concentrate (PC) would aid management of cases requiring transfused platelets (PLTs), where adminstration of whole-blood or platelet-rich plasma (PRP) might be contraindicated.
To test and validate a method for production of an equine PRP-PC product.
Six healthy Thoroughbred geldings from a research herd.
In this prospective experimental study, whole blood was collected and processed through multiple centrifugation steps to yield 120 mL of PC. The PC was stored at 22°C and gently and continuously agitated. Measurements of PLT count, pH, and concentrations of glucose, lactate, electrolytes, lactate dehydrogenase (LDH), and aspartate aminotransferase (AST), as well as partial pressures of oxygen and carbon dioxide were performed on days 0, 1, 2, 3, 5, and 7. Platelet aggregometry and bacterial culture were also performed.
The PC always had a PLT count of ≥550 × 10 cells/μL. Aggregometry graph amplitude (P < .0001) and area under the curve (P < .05) significantly decreased over time. Sodium, chloride, lactate (P < .0001), and oxygen (P < .01) concentrations significantly increased over time. pH (P < .001), glucose and bicarbonate concentrations (P < .0001) significantly decreased over time. There was no significant difference in potassium concentration, PLT count, LDH and AST activities and no bacterial growth from culture.
The described technique yielded a PC that meets the standards of the American Association of Blood Banks for human PC.
马血小板浓缩物（PC）的研发将有助于管理需要输注血小板（PLT）的病例，在这些病例中，输注全血或富含血小板血浆（PRP）可能是禁忌的。
测试并验证一种生产马PRP-PC产品的方法。
来自一个研究马群的6匹健康纯种阉马。
在这项前瞻性实验研究中，采集全血并通过多次离心步骤进行处理，以获得120 mL的PC。将PC储存在22°C下，并轻轻持续搅拌。在第0、1、2、3、5和7天对PLT计数、pH值、葡萄糖、乳酸、电解质、乳酸脱氢酶（LDH）和天冬氨酸转氨酶（AST）的浓度以及氧和二氧化碳分压进行测量。还进行了血小板聚集测定和细菌培养。
PC的PLT计数始终≥550×10⁹个细胞/μL。随着时间的推移，聚集测定图的振幅（P＜0.0001）和曲线下面积（P＜0.05）显著降低。钠、氯（P＜0.0001）、乳酸（P＜0.0001）和氧（P＜0.01）的浓度随着时间的推移显著增加。pH值（P＜0.001）、葡萄糖和碳酸氢盐浓度（P＜0.0001）随着时间的推移显著降低。钾浓度、PLT计数、LDH和AST活性没有显著差异，培养物中也没有细菌生长。
所描述的技术产生了一种符合美国血库协会人类PC标准的PC。
