Anderson D J, Johnson P M, Alexander N J, Jones W R, Griffin P D
J Reprod Immunol. 1987 Mar;10(3):231-57. doi: 10.1016/0165-0378(87)90089-1.
Two WHO-sponsored workshops were recently held to obtain a consensus view from researchers active in the field of reproductive immunology on the current status of the application of monoclonal antibodies to studies of molecular events underlying reproduction and to determine the feasibility of using this approach to identify trophoblast- or sperm-specific antigens that might represent suitable candidates for the development of antifertility vaccines. A total of 66 mouse monoclonal antibodies reacting with human sperm and 45 monoclonal antibodies reacting with human trophoblast membrane components were submitted by 29 laboratories. These were evaluated in coded form by 42 laboratories with the appropriate expertise in biochemistry, immunohistology and tests of reproductive cell function. The majority of both anti-sperm and anti-trophoblast monoclonal antibodies cross-reacted with cellular elements in non-reproductive tissues. However, at least five monoclonal antibodies (two anti-trophoblast and three anti-sperm) appeared to demonstrate sufficient specificity to warrant further investigation as reagents for the identification of antifertility vaccine candidates.
最近举办了两场由世界卫生组织赞助的研讨会，目的是让活跃于生殖免疫学领域的研究人员就单克隆抗体在生殖相关分子事件研究中的应用现状达成共识，并确定使用这种方法来识别可能成为抗生育疫苗开发合适候选物的滋养层或精子特异性抗原的可行性。29个实验室提交了总共66种与人类精子反应的小鼠单克隆抗体以及45种与人类滋养层膜成分反应的单克隆抗体。42个具备生物化学、免疫组织学和生殖细胞功能检测等相关专业知识的实验室对这些抗体进行了编码形式的评估。大多数抗精子和抗滋养层单克隆抗体都与非生殖组织中的细胞成分发生交叉反应。然而，至少有五种单克隆抗体（两种抗滋养层抗体和三种抗精子抗体）似乎表现出了足够的特异性，值得作为识别抗生育疫苗候选物的试剂作进一步研究。
