Institute for Molecular Biology and Genetics, Center for Fungal Pathogenesis, Chonbuk National University, Chonbuk 561-756, Republic of Korea.
J Microbiol. 2011 Aug;49(4):680-3. doi: 10.1007/s12275-011-1022-4. Epub 2011 Sep 2.
UV-mutagenesis was performed to obtain mutant strains that demonstrate altered production of phleichrome, a secondary metabolite of Cladosporium phlei. Among fifty mutants selected, based on the increased area and intensity of the purple pigment surrounding the colonies, the strain M0035 showed the highest production of phleichrome, more than seven fold over wild type. Plate cultures of the M0035 strain resulted in a total of 592 mg phleichrome consisting of 146 mg and 446 mg from the mycelia and agar media, respectively. The M0035 strain displayed a growth rate and a mycelial mass comparable to the parental strain but had significantly reduced asexual sporulation.
进行了 UV 诱变以获得突变株，这些突变株表现出变色素的产生发生改变，变色素是草生枝孢的次级代谢产物。在根据菌落周围紫色色素的面积和强度增加而选择的五十个突变株中，M0035 菌株显示出最高的变色素产量，比野生型高出七倍以上。M0035 菌株的平板培养总共产生了 592mg 的变色素，其中菌丝和琼脂培养基分别产生了 146mg 和 446mg。M0035 菌株的生长速度和菌丝体质量与亲本菌株相当，但无性孢子形成显著减少。
