Bobrovnyk S A, Demchenko M A, Komysarenko S V
Ukr Biokhim Zh (1999). 2011 May-Jun;83(3):58-64.
The features of monovalent and bivalent binding of receptors (or antibodies) with a polyvalent ligand (or with an antigen) are considered. It is shown that the rigid connection of the binding sites of the receptor brings to high increase of binding affinity for the corresponding ligand, but only in case if its epitopes are fully complementary to both sites of the receptor binding. If not, then there is no advantage of the binding of bivalent receptor before univalent binding. If the binding sites of the receptor are connected by a flexible linker, then regardless of location of epitopes of the corresponding ligand there is the successful fastening of receptor and ligand. Exactly the connection by a flexible linker is used by Nature in most cases at constructing of polyvalent receptors.
本文考虑了受体（或抗体）与多价配体（或抗原）的单价和二价结合特征。结果表明，受体结合位点的刚性连接可使对相应配体的结合亲和力大幅提高，但前提是其表位与受体结合的两个位点完全互补。否则，二价受体结合并不比单价结合更具优势。如果受体的结合位点通过柔性接头连接，那么无论相应配体表位的位置如何，受体与配体都能成功结合。实际上，自然界在构建多价受体时大多采用柔性接头连接。
