Dall'Aglio Cecilia, Zannoni Augusta, Mercati Francesca, Forni Monica, Bacci Maria Laura, Boiti Cristiano
Dipartimento di Scienze Biopatologiche Veterinarie ed Igiene delle Produzioni Animali e Alimentari, Università degli Studi di Perugia, Via S. Costanzo 4, 06126 Perugia, Italy.
Regul Pept. 2011 Dec 10;172(1-3):51-7. doi: 10.1016/j.regpep.2011.08.007. Epub 2011 Sep 5.
The aim of the present work was to characterize the expression and cell type distribution of the orexinic system in the major swine salivary glands by RT-PCR and immunohistochemistry techniques. Tissue samples of the mandibular (MSG), sub-lingual, and parotid glands were obtained from six pigs. Marked differences in the expression of the orexinergic system were observed among the salivary glands. Prepro-orexin (PPOX) mRNA abundance was approximately 15-fold higher (P<0.05) in MSG than in the others salivary glands. The expression level of OX1R mRNA did not differ among the salivary glands, while that for OX2R transcript was undetectable. The hypothalamus, used as positive control tissues, showed the highest expression level for each component of the orexinic system. Immunoreactivity (IR) for both orexins A and B (OXA and OXB), and cognate receptors (OX1R and OX2R) were identified only in the excretory striated ducts of the MSG while acinar cells were not immunoreactive. Both sub-lingual and parotid glands completely lacked IR for any component of the orexinergic system. In the MSG, parasympathetic neurons and axons of local salivary gland ganglia were IR to both OXA and OXB and also to their receptors.
本研究的目的是通过逆转录聚合酶链反应（RT-PCR）和免疫组织化学技术，对主要猪唾液腺中食欲素系统的表达和细胞类型分布进行表征。从六头猪身上获取下颌下腺（MSG）、舌下腺和腮腺的组织样本。在唾液腺之间观察到食欲素能系统表达存在显著差异。MSG中前食欲素原（PPOX）mRNA丰度比其他唾液腺高约15倍（P<0.05）。唾液腺中OX1R mRNA的表达水平没有差异，而OX2R转录本的表达水平未检测到。用作阳性对照组织的下丘脑显示食欲素系统各成分的表达水平最高。仅在MSG的排泄纹状管中鉴定出食欲素A和B（OXA和OXB）以及同源受体（OX1R和OX2R）的免疫反应性（IR），而腺泡细胞无免疫反应性。舌下腺和腮腺对食欲素能系统的任何成分均完全缺乏IR。在MSG中，副交感神经元和局部唾液腺神经节的轴突对OXA和OXB及其受体均呈IR。
