Laboratoire de Biogénotoxicologie et Mutagenèse Environnementale, Fédération de Recherche CNRS 3098 ECCOREV, Facultés de Médecine et Pharmacie, Université de Méditerranée, Marseille, France.
Fertil Steril. 2011 Oct;96(4):1002-7. doi: 10.1016/j.fertnstert.2011.07.1106. Epub 2011 Sep 3.
To assess the genotoxicity of 1,2-propanediol (PrOH) on mouse oocytes by comet assay.
In vitro assay using murine model.
Biogenotoxicology research laboratory.
ANIMAL(S): CD1 female mice.
INTERVENTION(S): Three 40-oocyte groups were exposed to different PrOH concentrations (5%, 7.5%, and 15%). Each concentration was tested during both long and short exposures (1-2 hours and 1-5 minutes) in comparison with control groups. DNA damage was evaluated by a single-cell gel electrophoresis assay, also called "comet assay," and analyzed with Komet software.
MAIN OUTCOME MEASURE(S): DNA damage was quantified as Olive tail moment (OTM). Interpretation was done on OTM with the use of χ(2).
RESULT(S): High PrOH concentrations (7.5% and 15%) induced significant DNA damage on mouse oocytes. The OTM χ(2) values were 4.16 ± 0.40 and 6.80 ± 0.4 with 7.5% PrOH at 1 and 2 hours, respectively, 24.35 ± 1.60 with 15% at 1 hour, and for 2h at 15% the DNA damage was too drastic to calculate OTM χ(2). After 1 and 5 minutes, the OTM χ(2) values were, respectively, 5.19 ± 0.26 and 6.06 ± 0.42 with 7.5%, and 7.53 ± 0.33 and 16.81 ± 0.67 with 15%.
CONCLUSION(S): High concentrations of PrOH (7.5% and 15%) induced significant DNA damage on mouse oocytes, whatever the exposure duration. These results should be interpreted with caution, because additional data are needed to evaluate PrOH genotoxicity and DNA oocyte reparation after exposure to high PrOH concentrations.
通过彗星试验评估 1,2-丙二醇（PrOH）对小鼠卵母细胞的遗传毒性。
使用鼠模型的体外试验。
生物遗传毒性学研究实验室。
CD1 雌性小鼠。
三组 40 个卵母细胞组分别暴露于不同浓度的 PrOH（5%、7.5%和 15%）下。每个浓度均在长暴露（1-2 小时）和短暴露（1-5 分钟）下进行测试，并与对照组进行比较。DNA 损伤通过单细胞凝胶电泳试验（也称为“彗星试验”）进行评估，并使用 Komet 软件进行分析。
DNA 损伤程度用橄榄尾巴矩（OTM）量化。采用 χ(2) 检验对 OTM 进行解释。
高浓度 PrOH（7.5%和 15%）导致小鼠卵母细胞 DNA 损伤显著增加。7.5%PrOH 在 1 小时和 2 小时时，OTM χ(2) 值分别为 4.16±0.40 和 6.80±0.4；15%PrOH 在 1 小时时，OTM χ(2) 值为 24.35±1.60；而在 2 小时时，15%PrOH 导致 DNA 损伤过于严重而无法计算 OTM χ(2) 值。1 分钟和 5 分钟后，7.5%PrOH 的 OTM χ(2) 值分别为 5.19±0.26 和 6.06±0.42；而 15%PrOH 的 OTM χ(2) 值分别为 7.53±0.33 和 16.81±0.67。
高浓度的 PrOH（7.5%和 15%）无论暴露时间长短，均导致小鼠卵母细胞 DNA 损伤显著增加。这些结果应谨慎解释，因为需要更多数据来评估高浓度 PrOH 暴露后 PrOH 的遗传毒性和卵母细胞 DNA 的修复情况。
