Department of OB/GYN, University of Michigan, Ann Arbor, MI 48108, USA.
J Assist Reprod Genet. 2013 Jul;30(7):865-75. doi: 10.1007/s10815-013-0028-8. Epub 2013 Jun 19.
To highlight emerging techniques aimed at improving oocyte cryopreservation.
Review of available and relevant literature through Pubmed and Medline searches.
Oocyte cryopreservation is an increasingly common procedure utilized for assisted reproduction and may benefit several patient populations. Therefore, improving efficiency is paramount in realizing the tremendous promise of this approach. However, in addition to numerous studies looking to improve oocyte cryopreservation efficacy via examination of variables involved with protocol methodology, such as type/concentration of cryoprotectant (CPA), type of storage device, or cooling/warming rates, there are more novel approaches for improvement. These alternate approaches include utilizing different the stages of oocytes, examining alteration of basal media and buffer composition, optimizing CPA exchange protocols and device loading through use of automated technology, as well as examination/manipulation of oocyte cellular composition to improve cryotolerance. Finally, elucidating more accurate or insightful indicators of "success" is crucial for continued improvement of oocyte cryopreservation.
Oocyte cryopreservation has improved dramatically in recent years and is receiving widespread clinical use. Novel approaches to further improve success, as well as improved methods to assess this success will aid in continued improvement.
强调旨在提高卵母细胞冷冻保存效果的新兴技术。
通过 Pubmed 和 Medline 搜索，对现有相关文献进行综述。
卵母细胞冷冻保存是一种越来越常见的辅助生殖技术，可能使许多患者群体受益。因此，提高效率是实现这一方法巨大潜力的关键。然而，除了许多研究通过检查与方案方法相关的变量来提高卵母细胞冷冻保存效果，例如冷冻保护剂（CPA）的类型/浓度、储存设备的类型或冷却/升温速率外，还有更多改进的新方法。这些替代方法包括利用卵母细胞的不同阶段，检查基础培养基和缓冲液组成的改变，通过使用自动化技术优化 CPA 交换方案和设备加载，以及检查/操作卵母细胞的细胞成分以提高抗冻性。最后，阐明更准确或有见地的“成功”指标对于卵母细胞冷冻保存的持续改进至关重要。
近年来，卵母细胞冷冻保存技术有了显著提高，并得到了广泛的临床应用。进一步提高成功率的新方法，以及改进评估成功的方法，将有助于持续改进。
