CRA-GPG (Consiglio per la Ricerca e Sperimentazione in Agricoltura-Centro di Ricerca per la Genomica e la PostGenomica), Genomic Research Centre, Via S Protaso 302, 29017 Fiorenzuola D'Arda (Piacenza) Italy.
J Anim Sci. 2012 Jan;90(1):16-26. doi: 10.2527/jas.2010-3706. Epub 2011 Sep 2.
In this study, the effect of polymorphisms in the leptin gene on the hematological variables in periparturient dairy cows was investigated. The hematological profile of 67 Holstein cows was assessed for 6 wk around calving. The DNA of the cows was genotyped at 6 polymorphic loci within the leptin gene, and 7 haplotypes were reconstructed. Significant haplotype substitution effects were found, for haplotype 1, on total white blood cell count for 2 wk around calving (+0.70 10(3)/μL, P = 0.05; +1.38 10(3)/μL, P = 0.0001); on neutrophil cell count in the first week after calving (+0.94 10(3)/μL, P = 0.001); on lymphocyte count during the 3 wk before and the first week after calving (+0.32 10(3)/μL, P = 0.05; +0.27 10(3)/μL, P = 0.03; +0.26 10(3)/μL, P = 0.04; +0.34 10(3)/μL, P = 0.01); on red blood cell count during the last week before calving and wk 1 and 2 after calving (+0.21 10(6)/μL, P = 0.02; +0.23 10(6)/μL, P = 0.01; +0.20 10(6)/μL, P = 0.03); on mean corpuscular volume (-1.35 fL, P = 0.01; -1.29 fL, P = 0.002; -1.18 fL, P = 0.004; -1.09 fL, P = 0.008; -1.23 fL, P = 0.003; -1.31 fL, P = 0.003); and on mean corpuscular hemoglobin (-0.37 pg, P = 0.05; -0.38 pg, P = 0.02; -0.39 pg, P = 0.01; -0.34 pg, P = 0.03; -0.40 pg, P = 0.01; -0.40 pg, P = 0.01) during all 6 wk analyzed. Significant haplotype substitution effects, but opposite those of haplotype-1, were found for haplotype-2 on white blood cell count (-1.10 10(3)/μL, P = 0.01; -1.30 10(3)/μL, P = 0.002; -1.09 10(3)/μL, P = 0.01) and neutrophil count (-0.82 10(3)/μL, P = 0.02; -0.95 10(3)/μL, P = 0.005; -0.92 10(3)/μL, P = 0.01). Haplotype-3 influenced red blood cell count (-0.23 10(6)/μL, P = 0.03; -0.28 10(6)/μL, P = 0.01; -0.34 10(6)/μL, P = 0.002) during the last 2 wk before and the first week after calving, and also, with effects evident only in wk 3 and 2 before calving, mean corpuscular volume (+1.38 fL, P = 0.03; +0.97 fL, P = 0.05; +1.08 fL, P = 0.05), mean corpuscular hemoglobin (+0.58 pg, P = 0.02; +0.38 pg, P = 0.04; 0.51 pg, P = 0.01), and red blood cell distribution width (-0.56% P = 0.02; -0.47%, P = 0.05). The current study provided evidence that several polymorphisms in the leptin gene play a role in the variability of hematological variables during the peripartum period, and might be used as genetic markers for improving the immunological conditions of dairy cows in critical productive periods.
在这项研究中，研究了瘦素基因多态性对围产期奶牛血液学变量的影响。在分娩前后的 6 周内，评估了 67 头荷斯坦奶牛的血液学特征。对奶牛的 DNA 在瘦素基因的 6 个多态性位点进行了基因分型，并重建了 7 个单倍型。发现了显著的单倍型替代效应，对于单倍型 1，在分娩前后的 2 周内，总白细胞计数增加了 0.70×10(3)/μL(P=0.05)；在分娩后的第一周内，中性粒细胞计数增加了 0.94×10(3)/μL(P=0.001)；淋巴细胞计数在分娩前 3 周和分娩后第一周增加了 0.32×10(3)/μL(P=0.05)；在分娩前最后一周和产后第 1、2 周，红细胞计数增加了 0.21×10(6)/μL(P=0.02)；在分娩前最后一周和产后第 1、2 周，红细胞平均体积减少了 1.35 fL(P=0.01)；在所有 6 周分析期间，平均红细胞血红蛋白减少了 0.37 pg(P=0.05)。对于单倍型 2，也发现了显著的单倍型替代效应，但与单倍型 1 的相反，白细胞计数减少了 1.10×10(3)/μL(P=0.01)；在分娩前的最后 2 周和分娩后的第一周，中性粒细胞计数减少了 0.82×10(3)/μL(P=0.02)；在分娩前的最后 2 周和分娩后的第一周，红细胞平均体积增加了 0.23×10(6)/μL(P=0.03)；在分娩前的最后 2 周和分娩后的第一周，平均红细胞血红蛋白增加了 0.58 pg(P=0.02)；在分娩前的最后 3 周和第 2 周，红细胞分布宽度减少了 0.56%(P=0.02)。本研究表明，瘦素基因的几个多态性在围产期血液学变量的变异性中起作用，并且可以作为改善奶牛在关键生产期免疫状况的遗传标记。
