钩端螺旋体的rRNA基因限制性酶切图谱：一种分子分型系统。

rRNA gene restriction patterns of Leptospira: a molecular typing system.

作者信息

Pérolat P, Grimont F, Regnault B, Grimont P A, Fournié E, Thevenet H, Baranton G

机构信息

Unité des Leptospires, WHO Collaborating Centre for Leptospirosis, Paris.

出版信息

Res Microbiol. 1990 Feb;141(2):159-71. doi: 10.1016/0923-2508(90)90025-l.

DOI:10.1016/0923-2508(90)90025-l

PMID:2189169

Abstract

A total of 67 serovar reference strains and 7 isolates belonging to the genus Leptospira were characterized by ribosomal ribonucleic acid (rRNA) gene restriction patterns. Fifty patterns were observed. Strains belonging to different genomic species always gave different patterns. However, genomic species were subdivided into several patterns. Forty-three serovars gave a specific pattern. Some serovars could not be separated by rRNA gene restriction patterns: strains of serovars icterohaemorrhagiae, copenhageni, lai, pyrogenes and jalna gave pattern 1; serovars birkini, mankarso and wolffi gave pattern 4; serovars canicola, gem, hebdomadis, pomona and hardjo (strain hardjoprajitno) gave pattern 12; serovars valbuzzi and zanoni gave pattern 14; serovars jonsis, malaya and sumneri gave pattern 16; serovars arborea, ballum, castellonis and kenya gave pattern 35; and serovars borincana and shermani gave pattern 43. These data provide the bases for a molecular typing system for the genus Leptospira.

摘要

对总共67株钩端螺旋体血清型参考菌株和7株钩端螺旋体属分离株进行了核糖体核糖核酸（rRNA）基因限制性酶切图谱分析。共观察到50种图谱。属于不同基因组种的菌株总是呈现不同的图谱。然而，基因组种又可细分为几种图谱。43个血清型呈现特定的图谱。一些血清型无法通过rRNA基因限制性酶切图谱区分：黄疸出血型、哥本哈根型、赖型、致热型和贾纳型菌株呈现图谱1；伯金尼型、曼卡索型和沃尔菲型血清型呈现图谱4；犬型、宝石型、七日热型、波摩那型和哈焦型（哈焦普拉吉诺菌株）呈现图谱12；瓦尔布齐型和扎诺尼型血清型呈现图谱14；琼西斯型、马来亚型和萨姆纳型血清型呈现图谱16；树栖型、巴鲁姆型、卡斯泰洛尼型和肯尼亚型血清型呈现图谱35；博林卡纳型和谢尔曼型血清型呈现图谱43。这些数据为钩端螺旋体属的分子分型系统提供了依据。