de Caballero O L, Dias Neto E, Koury M C, Romanha A J, Simpson A J
Centro de Pesquisas René Rachou, FIOCRUZ, Minas Gerais, Brazil.
J Clin Microbiol. 1994 May;32(5):1369-72. doi: 10.1128/jcm.32.5.1369-1372.1994.
Primers proposed for the diagnosis of the pathogenic spirochete Leptospira spp. (C. Gravekamp, H. V. D. Kemp, M. Franzen, D. Carrington, G.J. Schoone, G.J.J.M. Van Eys, C. O. R. Everard, R.A. Hartskeel, and W.J. Terpstra, J. Gen. Microbiol. 139:1691-1700, 1993) have been found to produce complex serovar-specific patterns under low-stringency PCR conditions. Such patterns obtained by low-stringency PCR, which maintain the specific band as an internal control, offer, an approach to the standardized identification of Leptospira serovars in clinical laboratories.
已发现为诊断致病性螺旋体钩端螺旋体属物种而设计的引物(C. 格拉韦坎普、H. V. D. 肯普、M. 弗兰岑、D. 卡林顿、G.J. 肖内、G.J.J.M. 范艾伊斯、C. O. R. 埃弗拉德、R.A. 哈茨基尔和W.J.特普斯特拉,《普通微生物学杂志》139:1691 - 1700, 1993)在低严谨度PCR条件下会产生复杂的血清型特异性图谱。通过低严谨度PCR获得的此类图谱,将特定条带作为内部对照保留,为临床实验室中钩端螺旋体血清型的标准化鉴定提供了一种方法。