Department of pharmacology, College of pharmacy; King Saud University, P,O, Box 2457, Riyadh 11451, Kingdom of Saudi Arabia.
Virol J. 2013 Nov 20;10:343. doi: 10.1186/1743-422X-10-343.
Human papillomavirus (HPV) is associated with different malignancies but its role in the pathogenesis of ovarian cancer is controversial. This study investigated the prevalence, genotyping and physical state of HPV in ovarian cancer Saudi patients.
Hundred formalin fixed paraffin embedded (FFPE) ovarian carcinoma tissues and their normal adjacent tissues (NAT) were included in the study. HPV was detected by nested polymerase chain reaction (PCR) using degenerated HPVL1 consensus primer pairs MY09/MY11 and GP5+/GP6 + to amplify a broad spectrum of HPV genotypes in a single reaction. The HPV positive samples were further genotyped using DNA sequencing. The physical state of the virus was identified using Amplification of Papillomavirus Oncogene Transcripts (APOT) assay in the samples positive for HPV16 and/or HPV18.
High percentage of HPV (42%) was observed in ovarian carcinoma compared to 8% in the NAT. The high-risk HPV types 16, 18 and 45 were highly associated with the advanced stages of tumor, while low-risk types 6 and 11 were present in NAT. In malignant tissues, HPV-16 was the most predominant genotype followed by HPV-18 and -45. The percentage of viral integration into the host genome was significantly high (61.1%) compared to 38.9% episomal in HPV positive tumors tissues. In HPV18 genotype the percentage of viral integration was 54.5% compared to 45.5% episomal.
The high risk HPV genotypes in ovarian cancer may indicate its role in ovarian carcinogenesis. The HPV vaccination is highly recommended to reduce this type of cancer.
人乳头瘤病毒（HPV）与多种恶性肿瘤有关，但它在卵巢癌发病机制中的作用仍存在争议。本研究旨在调查 HPV 在沙特卵巢癌患者中的流行率、基因型和物理状态。
本研究纳入了 100 例福尔马林固定石蜡包埋（FFPE）卵巢癌组织及其正常相邻组织（NAT）。采用嵌套聚合酶链反应（PCR）检测 HPV，使用 HPV L1 保守引物对 MY09/MY11 和 GP5+/GP6+进行扩增，在单个反应中可扩增广泛的 HPV 基因型。HPV 阳性样本进一步采用 DNA 测序进行基因分型。使用 HPV16 和/或 HPV18 阳性样本中的扩增致癌基因 HPV 转录物（APOT）检测来鉴定病毒的物理状态。
与 NAT 中的 8%相比，卵巢癌中 HPV 的高比例（42%）。高危 HPV 型 16、18 和 45 与肿瘤的晚期高度相关，而低危型 6 和 11 存在于 NAT 中。在恶性组织中，HPV-16 是最主要的基因型，其次是 HPV-18 和 -45。与 HPV 阳性肿瘤组织中 38.9%的游离状态相比，病毒整合到宿主基因组中的比例明显较高（61.1%）。HPV-18 基因型中病毒整合的比例为 54.5%，而游离状态的比例为 45.5%。
卵巢癌中的高危 HPV 基因型可能表明其在卵巢癌发生中的作用。强烈建议接种 HPV 疫苗以降低这种癌症的发生风险。
