通过聚合酶链反应测定牛轮状病毒G血清型
Determination of bovine rotavirus G serotype by polymerase chain reaction.
作者信息
Mondal Anjan, Aich Ranjit, Majee Sharmila, Bannalikar Anilkumar Sadashivrao
机构信息
Department of Microbiology, West Bengal University of Animal and Fishery Sciences, Belgachia, Kolkata 700037, India.
出版信息
Trop Anim Health Prod. 2012 Apr;44(4):763-7. doi: 10.1007/s11250-011-9962-6. Epub 2011 Sep 3.
A total of 113 diarrheic samples comprising of 68 buffalo calves and 45 cow calves were screened by RNA-PAGE for the detection of presence of rotavirus. RNA-PAGE analysis of these samples revealed 11 (9.73%) was found positive for rotavirus. Out of 68 faecal samples of buffalo calves tested for viral gastroenteritis, 8 (11.76%) were found positive for rotavirus. Similarly, out of 45 faecal samples of cattle calves tested for viral gastroenteritis, 3 (6.66%) was found positive for rotavirus. Rotavirus-positive samples represented long electropherotype. All RNA-PAGE-positive faecal samples for rotavirus subjected to RT-PCR for VP7 gene, ten samples yielded a specific product of 1,013 bp of VP7 gene. All the PCR-positive samples of the present study were subjected to genotyping with primers for G6, G8 and G10 genotype. All positive samples showed G10 genotype. This indicates that G10 may be predominant genotype among bovine calves in Mumbai region in India.
总共113份腹泻样本（包括68份水牛犊样本和45份奶牛犊样本）通过RNA聚丙烯酰胺凝胶电泳（RNA-PAGE）进行检测，以确定是否存在轮状病毒。对这些样本的RNA-PAGE分析显示，11份（9.73%）样本轮状病毒检测呈阳性。在检测病毒性肠胃炎的68份水牛犊粪便样本中，8份（11.76%）轮状病毒检测呈阳性。同样，在检测病毒性肠胃炎的45份奶牛犊粪便样本中，3份（6.66%）轮状病毒检测呈阳性。轮状病毒阳性样本呈现长电泳型。所有轮状病毒RNA-PAGE阳性粪便样本均进行VP7基因的逆转录聚合酶链反应（RT-PCR），10份样本产生了1013 bp的VP7基因特异性产物。本研究所有PCR阳性样本均使用G6、G8和G10基因型引物进行基因分型。所有阳性样本均显示为G10基因型。这表明G10可能是印度孟买地区牛犊中的主要基因型。
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