Life Science and Engineering College, Northwest University for Nationalities, Lanzhou 730030, China.
J Virol Methods. 2013 Apr;189(1):36-40. doi: 10.1016/j.jviromet.2013.01.002. Epub 2013 Jan 11.
To investigate the epidemical characteristics and genotype distributions of bovine rotavirus (BRV) in China, 195 fecal samples were collected from calves with diarrhea in China. Fecal samples were detected for rotavirus A antigen using ELISA. The positive samples were screened for VP7 and VP4 by RT-PCR. G serotyping and P genotyping were conducted on 53 VP7 and VP4 positive samples using RT-PCR. The results showed that 82 samples were found positive for BRV. 752bp, 660bp and 285bp bands were amplified for G-typing. 478bp, 375bp and 361bp bands were amplified for P-typing. The G6 and G10 serotypes were 29 (54.7%) and 8 (15.1%) in positive samples for VP7. P[5] and P[11] genotypes were 28 (52.8%) and 10 (18.9%) in the positive samples for VP4. The main combinations of BRV G serotype and P genotype were G6P[5] (28.3%), G6P[5]P[11] (5.7%), G10P[5] (5.7%) and G10G6P[5] (5.7%), respectively. Other combinations (including untypable) of G serotype and P genotype were 54.6%. The dominant G serotype and P genotype were G6 and P[5] respectively. The predominant combination of G and P serotypes was G6P[5]. This has significance for establishing the preventive measures against diarrhea caused by group A rotaviruses in cattle.
为了研究中国牛轮状病毒（BRV）的流行特征和基因型分布，本研究从中国腹泻犊牛中采集了 195 份粪便样本。使用 ELISA 检测粪便样本中的轮状病毒 A 抗原。对 RT-PCR 阳性样本进行 VP7 和 VP4 检测。对 53 份 VP7 和 VP4 阳性样本进行 G 血清型和 P 基因型检测。结果显示，82 份样本为 BRV 阳性。G 型鉴定扩增出 752bp、660bp 和 285bp 条带。P 型鉴定扩增出 478bp、375bp 和 361bp 条带。VP7 阳性样本中 G6 和 G10 血清型分别为 29（54.7%）和 8（15.1%）。VP4 阳性样本中 P[5]和 P[11]基因型分别为 28（52.8%）和 10（18.9%）。BRV G 血清型和 P 基因型的主要组合分别为 G6P[5]（28.3%）、G6P[5]P[11]（5.7%）、G10P[5]（5.7%）和 G10G6P[5]（5.7%）。其他组合（包括未定型）的 G 血清型和 P 基因型分别为 54.6%。优势 G 血清型和 P 基因型分别为 G6 和 P[5]。G 和 P 血清型的主要组合为 G6P[5]。这对于建立牛群 A 型轮状病毒引起的腹泻预防措施具有重要意义。
