Enteric Viruses Group, National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411 001, India.
Vet Microbiol. 2011 Mar 24;148(2-4):384-8. doi: 10.1016/j.vetmic.2010.08.027. Epub 2010 Sep 8.
A total of 78 fecal specimens were collected from both apparently healthy (n=71) and diarrheic (n=7) cattle from an organized farm in Pune, western India in December 2007-January 2008. Three specimens tested positive for group A rotavirus (RV) by antigen capture ELISA were subjected to RT-PCR for amplification of entire coding regions of three structural (VP4, VP6 and VP7) and one nonstructural (NSP4) genes. All three strains were genotyped as G8P[14]. Phylogenetic analysis of the VP7 and VP4 genes showed clustering of the VP7 gene with G8 strains of bovine origin and VP4 gene with P[14] strains of human origin. The identification of VP6 and NSP4 genes to have I2 (subgroup I) and E2 (genotype A) specificity, respectively of bovine and human origin indicated independent segregation of genes in bovine RV strains. This study indicates circulation of a rare RV genotype, G8P[14] in western India. To our knowledge, this is the second report on RV G8[14] isolated from bovine species after bovine group A RV strain, SUN9 from Japan.
2007 年 12 月至 2008 年 1 月，从印度西部浦那的一个组织农场的健康（n=71）和腹泻（n=7）牛中总共收集了 78 份粪便标本。通过抗原捕获 ELISA 检测，有 3 份标本对 A 组轮状病毒（RV）呈阳性，通过 RT-PCR 扩增了三个结构（VP4、VP6 和 VP7）和一个非结构（NSP4）基因的整个编码区。所有三种毒株均被鉴定为 G8P[14]。VP7 和 VP4 基因的系统进化分析显示，VP7 基因与牛源 G8 株聚类，VP4 基因与人源 P[14]株聚类。VP6 和 NSP4 基因鉴定为 I2（亚组 I）和 E2（基因型 A）特异性，分别来自牛和人源，表明牛 RV 株中基因的独立分离。本研究表明，在印度西部存在一种罕见的 RV 基因型 G8P[14]。据我们所知，这是继日本 SUN9 牛 A 组 RV 株之后从牛种分离出的第二种 RV G8[14]报告。
