Department of Geriatric Dentistry, School and Hospital of Stomatology, Peking University, Beijing, People's Republic of China.
Biomed Mater. 2011 Oct;6(5):055008. doi: 10.1088/1748-6041/6/5/055008. Epub 2011 Sep 5.
In recent years, interest in magnetic biomimetic scaffolds for tissue engineering has increased considerably. The aim of this study is to develop magnetic biodegradable fibrous materials with potential use in bone regeneration. Magnetic biodegradable Fe(3)O(4)/chitosan (CS)/poly vinyl alcohol (PVA) nanofibrous membranes were achieved by electrospinning with average fiber diameters ranging from 230 to 380 nm and porosity of 83.9-85.1%. The influences of polymer concentration, applied voltage and Fe(3)O(4) nanoparticles loading on the fabrication of nanofibers were investigated. The polymer concentration of 4.5 wt%, applied voltage of 20 kV and Fe(3)O(4) nanoparticles loading of lower than 5 wt% could produce homogeneous, smooth and continuous Fe(3)O(4)/CS/PVA nanofibrous membranes. X-ray diffraction (XRD) data confirmed that the crystalline structure of the Fe(3)O(4), CS and PVA were maintained during electrospinning process. Fourier transform infrared spectroscopy (FT-IR) demonstrated that the Fe(3)O(4) loading up to 5 wt% did not change the functional groups of CS/PVA greatly. Transmission electron microscopy (TEM) showed islets of Fe(3)O(4) nanoparticles evenly distributed in the fibers. Weak ferrimagnetic behaviors of membranes were revealed by vibrating sample magnetometer (VSM) test. Tensile test exhibited Young's modulus of membranes that were gradually enhanced with the increase of Fe(3)O(4) nanoparticles loading, while ultimate tensile stress and ultimate strain were slightly reduced by Fe(3)O(4) nanoparticles loading of 5%. Additionally, MG63 human osteoblast-like cells were seeded on the magnetic nanofibrous membranes to evaluate their bone biocompatibility. Cell growth dynamics according to MTT assay and scanning electron microscopy (SEM) observation exhibited good cell adhesion and proliferation, suggesting that this magnetic biodegradable Fe(3)O(4)/CS/PVA nanofibrous membranes can be one of promising biomaterials for facilitation of osteogenesis.
近年来，人们对用于组织工程的磁性仿生支架的兴趣大大增加。本研究旨在开发具有潜在骨再生应用的磁性可生物降解纤维材料。通过静电纺丝制备了平均纤维直径为 230-380nm、孔隙率为 83.9-85.1%的磁性可生物降解 Fe(3)O(4)/壳聚糖 (CS)/聚乙烯醇 (PVA) 纳米纤维膜。研究了聚合物浓度、施加电压和 Fe(3)O(4)纳米粒子负载对纳米纤维制备的影响。聚合物浓度为 4.5wt%、施加电压为 20kV、Fe(3)O(4)纳米粒子负载低于 5wt%时，可制备出均匀、光滑、连续的 Fe(3)O(4)/CS/PVA 纳米纤维膜。X 射线衍射 (XRD) 数据证实，在静电纺丝过程中，Fe(3)O(4)、CS 和 PVA 的晶体结构得以保持。傅里叶变换红外光谱 (FT-IR) 表明，Fe(3)O(4)的负载量高达 5wt%时，CS/PVA 的官能团没有发生明显变化。透射电子显微镜 (TEM) 显示，Fe(3)O(4)纳米粒子均匀分布在纤维中。振动样品磁强计 (VSM) 测试表明，薄膜具有较弱的铁磁性。拉伸试验表明，随着 Fe(3)O(4)纳米粒子负载量的增加，薄膜的杨氏模量逐渐增大，而当 Fe(3)O(4)纳米粒子负载量为 5%时，薄膜的最大拉伸应力和最大应变略有降低。此外，将 MG63 人成骨样细胞接种在磁性纳米纤维膜上，评价其骨生物相容性。根据 MTT 检测和扫描电子显微镜 (SEM) 观察的细胞生长动力学表明，细胞具有良好的黏附性和增殖性，这表明这种磁性可生物降解的 Fe(3)O(4)/CS/PVA 纳米纤维膜可以成为促进成骨的有前途的生物材料之一。
