Centre for Nanofibers & Nanotechnology, NUSNNI, Faculty of Engineering, National University of Singapore, Singapore.
J Mater Sci Mater Med. 2012 Jun;23(6):1511-9. doi: 10.1007/s10856-012-4630-6. Epub 2012 Apr 11.
Tissue engineering and nanotechnology have advanced a general strategy combining the cellular elements of living tissue with sophisticated functional biocomposites to produce living structures of sufficient size and function at a low cost for clinical relevance. Xylan, a natural polysaccharide was electrospun along with polyvinyl alcohol (PVA) to produce Xylan/PVA nanofibers for skin tissue engineering. The Xylan/PVA glutaraldehyde (Glu) vapor cross-linked nanofibers were characterized by SEM, FT-IR, tensile testing and water contact angle measurements to analyze the morphology, functional groups, mechanical properties and wettability of the fibers for skin tissue regeneration. The cell-biomaterial interactions were studied by culturing human foreskin fibroblasts on Xylan/PVA Glu vapor cross-linked and Xylan/PVA/Glu blend nanofibrous scaffolds. The observed results showed that the mechanical properties (72 %) and fibroblast proliferation significantly increased up to 23 % (P < 0.05) in 48 h Glu vapor cross-linked nanofibers compared to 24 h Glu vapor cross-linked Xylan/PVA nanofibers. The present study may prove that the natural biodegradable Xylan/PVA nanofibrous scaffolds have good potential for fibroblast adhesion, proliferation and cell matrix interactions relevant for skin tissue regeneration.
组织工程和纳米技术已经提出了一种通用策略，即将活组织的细胞成分与复杂的功能生物复合材料结合起来，以生产出具有足够大小和功能的活结构，同时成本低廉，具有临床相关性。木聚糖是一种天然多糖，与聚乙烯醇（PVA）一起进行静电纺丝，以生产用于皮肤组织工程的木聚糖/PVA 纳米纤维。通过 SEM、FT-IR、拉伸试验和水接触角测量对木聚糖/PVA 戊二醛（Glu）蒸汽交联纳米纤维进行了表征，以分析纤维的形态、官能团、机械性能和润湿性，用于皮肤组织再生。通过在木聚糖/PVA Glu 蒸汽交联和木聚糖/PVA/Glu 共混纳米纤维支架上培养人包皮成纤维细胞来研究细胞-生物材料相互作用。观察结果表明，与 24 小时 Glu 蒸汽交联的木聚糖/PVA 纳米纤维相比，机械性能（72%）和细胞增殖在 48 小时 Glu 蒸汽交联纳米纤维中显著增加了 23%（P<0.05）。本研究可能证明天然可生物降解的木聚糖/PVA 纳米纤维支架具有良好的成纤维细胞黏附、增殖和细胞基质相互作用的潜力，这与皮肤组织再生有关。
