Murakawa K, Kohno M, Yokokawa K, Yasunari K, Kurihara N, Hyono A, Takeda T
First Department of Internal Medicine, Osaka City University Medical School, Japan.
Am J Hypertens. 1990 Apr;3(4):307-9. doi: 10.1093/ajh/3.4.307.
We recently demonstrated that porcine endothelin increases intracellular calcium ion concentration ([Ca2+]i) in monolayers of cultured vascular smooth muscle cells isolated from rat renal arteries. The present study was designed to examine the effect of porcine endothelin on [Ca2+]i in the platelets of rats. [Ca2+]i was serially measured with the fluorescent dye, fura 2, and the photoprotein, aequorin. Endothelin (10(-9) mol/L, 10(-8) mol/L, and 10(-7) mol/L) did not induce any change in [Ca2+]i in the platelets of rats while thrombin (2 U/mL) dramatically increased [Ca2+]i as measured by these methods. Our results indicate that [Ca2+]i in rat platelets is not influenced by porcine endothelin.
我们最近证实，猪内皮素可增加从大鼠肾动脉分离的培养血管平滑肌细胞单层中的细胞内钙离子浓度（[Ca2+]i）。本研究旨在检测猪内皮素对大鼠血小板中[Ca2+]i的影响。使用荧光染料fura 2和光蛋白水母发光蛋白连续测量[Ca2+]i。内皮素（10(-9) mol/L、10(-8) mol/L和10(-7) mol/L）未引起大鼠血小板中[Ca2+]i的任何变化，而凝血酶（2 U/mL）通过这些方法测量可显著增加[Ca2+]i。我们的结果表明，大鼠血小板中的[Ca2+]i不受猪内皮素的影响。
