National Research Council Canada, Biotechnology Research Institute, Montreal, QC, Canada.
Appl Microbiol Biotechnol. 2012 Mar;93(6):2633-43. doi: 10.1007/s00253-011-3560-4. Epub 2011 Sep 6.
The effects of compost, nitrogen salts, and nitrogen-phosphorous-potassium (NPK) fertilizers on the methane oxidation potential (MOP) of landfill cover soil at various temperatures were assessed. For this, we used batch assays conducted at 5°C, 15°C, and 25°C with microcosms containing landfill cover soil slurries amended with these elements. Results indicated variable impacts dependent on the type of amendment and the incubation temperature. For a given incubation temperature, MOP varied from one compost to another and with the amount of compost added, except for the shrimp/peat compost. With this latter compost, independent of the amount, MOP values remained similar and were significantly higher than those obtained with other composts. Amendment with most of the tested nitrogen salts led to similar improvements in methanotrophic activity, except for urea. MOP with NPK fertilizer addition was amongst the highest in this study; the minimum value obtained with NPK (20-0-20) suggested the importance of P for methanotrophs. MOP generally increased with temperature, and nutrient limitation became less important at higher temperatures. Overall, at each of the three temperatures tested, MOP with NPK fertilizer amendments provided the best results and was comparable to those observed with the addition of the shrimp/peat compost. The results of this study provide the first evidence of the following: (1) compost addition to improve methanotrophic activity in a landfill cover soil should consider the amount and type of compost used and (2) the importance of using NPK fertilizers rather than nitrogen salts, in enhancing this activity, primarily at low temperatures. One can also consider the potential beneficial impact of adding these elements to enhance plant growth, which is an advantage for MOP.
评估了堆肥、氮盐和氮磷钾（NPK）肥料对不同温度下垃圾填埋覆盖土壤甲烷氧化潜能（MOP）的影响。为此，我们使用微宇宙进行了批处理实验，在 5°C、15°C 和 25°C 下，微宇宙中含有添加了这些元素的垃圾填埋覆盖土壤泥浆。结果表明，影响取决于添加物的类型和培养温度。对于给定的培养温度，MOP 因堆肥类型和添加量而异，而虾/泥炭堆肥则不同。对于后者，无论添加量多少，MOP 值都保持相似，且明显高于其他堆肥。大多数测试的氮盐的添加都导致甲烷营养活性相似的提高，除了尿素。添加 NPK 肥料的 MOP 在本研究中是最高的；用 NPK（20-0-20）添加获得的最小 MOP 值表明 P 对甲烷营养菌的重要性。MOP 通常随温度升高而增加，在较高温度下营养限制变得不那么重要。总的来说，在测试的三个温度中的每一个温度下，NPK 肥料添加的 MOP 提供了最佳结果，与添加虾/泥炭堆肥的结果相当。这项研究的结果提供了以下证据：（1）为了提高垃圾填埋覆盖土壤中的甲烷营养活性而添加堆肥应考虑使用的堆肥量和类型；（2）在提高这种活性方面，特别是在低温下，使用 NPK 肥料而不是氮盐更为重要。人们还可以考虑添加这些元素来增强植物生长的潜在有益影响，这对 MOP 是一个优势。
