Institute of Food Chemistry, University of Münster, Münster, Germany.
J Agric Food Chem. 2011 Oct 12;59(19):10470-6. doi: 10.1021/jf2026814. Epub 2011 Sep 20.
Fusarin C is a mycotoxin that is produced by a variety of Fusarium species and is therefore a possible contaminant in food and feed. For this reason, a reliable high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the determination of fusarin C in food and feed samples was developed based on dispersive solid phase extraction (DSPE). This method has a limit of detection (LOD) of 2 μg/kg, a limit of quantitation (LOQ) of 7 μg/kg, and a recovery rate of 80%. Fifty different corn samples were analyzed, and fusarin C was detected in 40 of them. The fusarin C level varied in kernels of corn ears from not detectable up to 83 mg/kg and in food samples from not detectable up to 28 μg/kg. The co-occurrence of further structural analogues of fusarin C was confirmed by high-performance liquid chromatography Fourier transformation mass spectrometry (HPLC-FTMS). In addition, the stability of fusarin C under storage conditions was evaluated.
伏马菌素 C 是一种真菌毒素，由多种镰刀菌属产生，因此可能会污染食品和饲料。基于分散固相萃取（DSPE），建立了一种用于检测食品和饲料中伏马菌素 C 的可靠高效液相色谱-串联质谱法（HPLC-MS/MS）。该方法的检测限（LOD）为 2μg/kg，定量限（LOQ）为 7μg/kg，回收率为 80%。分析了 50 个不同的玉米样品，其中 40 个样品检测到了伏马菌素 C。玉米穗的玉米粒中伏马菌素 C 的含量从无法检测到 83mg/kg 不等，食品样品中从无法检测到 28μg/kg 不等。通过高效液相色谱傅里叶变换质谱法（HPLC-FTMS）证实了伏马菌素 C 结构类似物的同时存在。此外，还评估了伏马菌素 C 在储存条件下的稳定性。
