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苹果消费的短期生物标志物。

Short-term biomarkers of apple consumption.

作者信息

Saenger Theresa, Hübner Florian, Humpf Hans-Ulrich

机构信息

Institute of Food Chemistry, Westfälische Wilhelms-Universität Münster, Münster, Germany.

出版信息

Mol Nutr Food Res. 2017 Mar;61(3). doi: 10.1002/mnfr.201600629. Epub 2016 Dec 20.

DOI:10.1002/mnfr.201600629
PMID:27794196
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6120132/
Abstract

SCOPE: Urinary biomarkers are used to estimate the nutritional intake of humans. The aim of this study was to distinguish between low, medium, and high apple consumption by quantifying possible intake biomarkers in urine samples after apple consumption by HPLC-MS/MS. Apples were chosen as they are the most consumed fruits in Germany. METHODS AND RESULTS: Thirty subjects took part in 7-day study. They abstained from apples and apple products except for one weighed apple portion resembling one, two, or four apples. Before apple consumption and during the following days spot urine samples were collected. These urine samples were incubated with β-glucuronidase, diluted, and directly measured by HPLC-MS/MS. Phloretin, epicatechin, procyanidin B2, and quercetin were detected in urine using Scheduled MRM mode. Phloretin was confirmed as a urinary biomarker of apple intake and had the ability to discriminate between low or medium (one or two apples) and high apple consumption (four apples). The groups also differ in the excretion of epicatechin and procyanidin B2. CONCLUSION: Apple consumption can be monitored by urinary biomarkers for a period of at least 12 h after consumption. Furthermore the amount of apples consumed can be estimated by the concentration of certain biomarkers.

摘要

范围：尿生物标志物用于评估人类的营养摄入情况。本研究的目的是通过高效液相色谱-串联质谱法（HPLC-MS/MS）对食用苹果后尿样中可能的摄入生物标志物进行定量分析，以区分低、中、高苹果摄入量。选择苹果是因为它们是德国消费最多的水果。 方法与结果：30名受试者参与了为期7天的研究。他们除了食用一份重量相当于一个、两个或四个苹果的苹果外， abstained from苹果和苹果制品。在食用苹果前及之后的几天内收集即时尿样。这些尿样与β-葡萄糖醛酸酶一起孵育、稀释，然后直接用HPLC-MS/MS进行测量。使用定时多反应监测模式在尿样中检测到根皮素、表儿茶素、原花青素B2和槲皮素。根皮素被确认为苹果摄入的尿生物标志物，并且有能力区分低或中等（一个或两个苹果）和高苹果摄入量（四个苹果）。这些组在表儿茶素和原花青素B2的排泄方面也存在差异。 结论：食用苹果后至少12小时内，可通过尿生物标志物监测苹果摄入量。此外，可通过某些生物标志物的浓度估计食用苹果的量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f85/6120132/e9d690bd4e87/MNFR-61-na-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f85/6120132/7a6391eba8c2/MNFR-61-na-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f85/6120132/623762637b03/MNFR-61-na-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f85/6120132/ff3a2d4f67c6/MNFR-61-na-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f85/6120132/50ad8faa23bf/MNFR-61-na-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f85/6120132/958f497cb3db/MNFR-61-na-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f85/6120132/e9d690bd4e87/MNFR-61-na-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f85/6120132/7a6391eba8c2/MNFR-61-na-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f85/6120132/623762637b03/MNFR-61-na-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f85/6120132/ff3a2d4f67c6/MNFR-61-na-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f85/6120132/50ad8faa23bf/MNFR-61-na-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f85/6120132/958f497cb3db/MNFR-61-na-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f85/6120132/e9d690bd4e87/MNFR-61-na-g006.jpg

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本文引用的文献

[1]
Systemic absorption and metabolism of dietary procyanidin B4 in pigs.

Mol Nutr Food Res. 2014-10-28

[2]
Dietary assessment methods in epidemiologic studies.

Epidemiol Health. 2014-7-22

[3]
Urine-an untapped goldmine for biomarker discovery?

Sci China Life Sci. 2013-12

[4]
Distribution and quantification of flavan-3-ols and procyanidins with low degree of polymerization in nuts, cereals, and legumes.

J Agric Food Chem. 2013-9-25

[5]
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Mol Nutr Food Res. 2012-4

[6]
Evaluation of flavonoids and enterolactone in overnight urine as intake biomarkers of fruits, vegetables and beverages in the Inter99 cohort study using the method of triads.

Br J Nutr. 2012-3-27

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A new high-performance liquid chromatography-tandem mass spectrometry method based on dispersive solid phase extraction for the determination of the mycotoxin fusarin C in corn ears and processed corn samples.

J Agric Food Chem. 2011-9-20

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J Agric Food Chem. 2011-9-12

[9]
Intestinal transit and systemic metabolism of apple polyphenols.

Eur J Nutr. 2010-12-24

[10]
Absorption, metabolism, and excretion of cider dihydrochalcones in healthy humans and subjects with an ileostomy.

J Agric Food Chem. 2009-3-11

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