Otto O, Steinbock L J, Wong D W, Gornall J L, Keyser U F
Cavendish Laboratory, University of Cambridge, Cambridge, United Kingdom.
Rev Sci Instrum. 2011 Aug;82(8):086102. doi: 10.1063/1.3623502.
We have developed optical tweezers, with force measurements based on fast video tracking, for analysis and control of DNA translocation through nanocapillaries. Nanocapillaries are single-molecule biosensors with very similar characteristics to solid-state nanopores. Our novel experimental setup allows for ionic-current measurements in which the nanocapillary is oriented perpendicular to the trapping laser. Using video-based particle tracking, we are able to measure the position of DNA coated colloids at sub-millisecond resolution and in real-time. We present the first electrophoretic force and simultaneous ionic-current measurements of a single DNA molecule inside the orifice of a nanocapillary.
我们开发了基于快速视频跟踪进行力测量的光镊，用于分析和控制DNA通过纳米毛细管的转运。纳米毛细管是与固态纳米孔具有非常相似特性的单分子生物传感器。我们新颖的实验装置允许进行离子电流测量，其中纳米毛细管垂直于捕获激光定向。通过基于视频的粒子跟踪，我们能够以亚毫秒分辨率实时测量涂有DNA的胶体的位置。我们展示了纳米毛细管孔内单个DNA分子的首次电泳力和同步离子电流测量。
