Tokyo University of Agriculture & Technology, Graduate School of Bioapplications and Science Engineering, Koganei, Tokyo 184-8588, Japan.
J Biomed Opt. 2011 Aug;16(8):086012. doi: 10.1117/1.3613929.
In order to visualize human skin hemodynamics, we investigated a method that is specifically developed for the visualization of concentrations of oxygenated blood, deoxygenated blood, and melanin in skin tissue from digital RGB color images. Images of total blood concentration and oxygen saturation can also be reconstructed from the results of oxygenated and deoxygenated blood. Experiments using tissue-like agar gel phantoms demonstrated the ability of the developed method to quantitatively visualize the transition from an oxygenated blood to a deoxygenated blood in dermis. In vivo imaging of the chromophore concentrations and tissue oxygen saturation in the skin of the human hand are performed for 14 subjects during upper limb occlusion at 50 and 250 mm Hg. The response of the total blood concentration in the skin acquired by this method and forearm volume changes obtained from the conventional strain-gauge plethysmograph were comparable during the upper arm occlusion at pressures of both 50 and 250 mm Hg. The results presented in the present paper indicate the possibility of visualizing the hemodynamics of subsurface skin tissue.
为了可视化人体皮肤血液动力学，我们研究了一种专门用于从数字 RGB 彩色图像中可视化含氧血液、去氧血液和皮肤组织中黑色素浓度的方法。也可以根据含氧血液和去氧血液的结果重建总血液浓度和氧饱和度图像。使用类似组织的琼脂凝胶体模型的实验证明了所开发方法能够定量可视化真皮中从含氧血液到去氧血液的转变。对 14 名受试者的手部皮肤中的色团浓度和组织氧饱和度进行了活体成像，在上肢闭塞时在 50 和 250 mmHg 下进行。在这两种压力下，通过该方法获得的皮肤总血液浓度的响应与从常规应变计体积描记器获得的前臂体积变化相当。本文介绍的结果表明了可视化皮下组织血液动力学的可能性。
