Nanobiophotonics and Laser Microspectroscopy Center, Interdisciplinary Research Institute in Bio-Nano-Sciences, Babes-Bolyai University, 42 T. Laurian Str., 400271 Cluj-Napoca, Romania.
Nanostructured Materials and Bio-Nano-Interfaces Center, Interdisciplinary Research Institute in Bio-Nano-Sciences, Babes-Bolyai University, 42 T. Laurian Str., 400271 Cluj-Napoca, Romania.
Int J Mol Sci. 2022 Nov 8;23(22):13724. doi: 10.3390/ijms232213724.
Photothermal therapy (PTT) is gaining a lot of interest as a cancer treatment option with minimal side effects due to the efficient photothermal agents employed. They are based on nanomaterials that, upon laser irradiation, absorb photon energy and convert it into heat to induce hyperthermia, which destroys the cancer cells. Here, the unique light-to-heat conversion features of three different gold nanotriangular nanoparticles (AuNTs) are evaluated with respect to their absorption properties to select the most efficient nanoheater with the highest potential to operate as an efficient photothermal agent. AuNTs with LSPR response in- and out- of resonance with the 785 nm near-infrared (NIR) excitation wavelength are investigated. Upon 15 min laser exposure, the AuNTs that exhibit a plasmonic response in resonance with the 785 nm laser line show the highest photothermal conversion efficacy of 80%, which correlates with a temperature increase of 22 °C. These photothermal properties are well-preserved in agarose-based skin biological phantoms that mimic the melanoma tumoral tissue and surrounding healthy tissue. Finally, in vitro studies on B16.F10 melanoma cells prove by fluorescence staining and MTT assay that the highest phototoxic effect after NIR laser exposure is induced by AuNTs with LSPR response in resonance with the employed laser line, thus demonstrating their potential implementation as efficient photothermal agents in PTT.
光热疗法（PTT）作为一种癌症治疗方法，由于采用了高效的光热剂，副作用极小，因此越来越受到关注。这些光热剂基于纳米材料，在激光照射下，纳米材料吸收光子能量并将其转化为热量，从而引发高热，杀死癌细胞。在这里，我们评估了三种不同的金纳米三角（AuNTs）的独特光热转换特性，以评估其吸收特性，从而选择最有效的纳米加热器，以实现作为高效光热剂的最大潜力。我们研究了在 785nm 近红外（NIR）激发波长的内和外共振处具有局域等离子体共振（LSPR）响应的 AuNTs。在 15 分钟的激光照射下，与 785nm 激光线产生等离子体响应的 AuNTs 表现出最高的光热转换效率为 80%，这与 22°C 的温度升高相对应。这些光热特性在琼脂糖基皮肤生物模拟物中得到了很好的保留，这些模拟物模拟了黑色素瘤肿瘤组织和周围健康组织。最后，体外 B16.F10 黑色素瘤细胞研究通过荧光染色和 MTT 测定证明，在近红外激光照射后，具有 LSPR 响应与所用激光线共振的 AuNTs 诱导的光毒性作用最大，从而证明了它们作为 PTT 中高效光热剂的潜在应用。
