Purdue University, Department of Physics, West Lafayette, Indiana 47907, USA.
J Biomed Opt. 2011 Aug;16(8):087004. doi: 10.1117/1.3615970.
Tissue dynamics spectroscopy uses digital holography as a coherence gate to extract depth-resolved quasi-elastic dynamic light scattering from inside multicellular tumor spheroids. The temporal speckle contrast provides endogenous dynamical images of proliferating and hypoxic or necrotic tissues. Fluctuation spectroscopy similar to diffusing wave spectroscopy is performed on the dynamic speckle to generate tissue-response spectrograms that track time-resolved changes in intracellular motility in response to environmental perturbations. The spectrograms consist of several frequency bands that range from 0.005 to 5 Hz. The fluctuation spectral density and temporal autocorrelations show the signature of constrained anomalous diffusion, but with large fluctuation amplitudes caused by active processes far from equilibrium. Differences in the tissue-response spectrograms between the proliferating outer shell and the hypoxic inner core differentiate normal from starved conditions. The differential spectrograms provide an initial library of tissue-response signatures to environmental conditions of temperature, osmolarity, pH, and serum growth factors.
组织动力学光谱学使用数字全息术作为相干门，从多细胞肿瘤球体内部提取深度分辨的准弹性动态光散射。时间散斑对比提供了增殖和缺氧或坏死组织的内源性动力学图像。在动态散斑上进行类似于扩散波光谱学的波动光谱学，以生成组织响应频谱图，跟踪细胞内运动对环境干扰的时间分辨变化。频谱图包含几个频率带，范围从 0.005 到 5 Hz。波动谱密度和时间自相关显示出受限异常扩散的特征，但由于远离平衡的主动过程，波动幅度较大。增殖外壳和缺氧内核之间的组织响应频谱图的差异将正常状态与饥饿状态区分开来。微分频谱图为温度、渗透压、pH 值和血清生长因子等环境条件提供了组织响应特征的初始库。
