Department of Biotechnology, Alagappa University, Karaikudi, Tamil Nadu, India.
Arch Microbiol. 2010 Oct;192(10):843-54. doi: 10.1007/s00203-010-0612-6. Epub 2010 Aug 10.
The aim of the work is to investigate the effect of marine bacterial culture supernatants on biofilm formation of Vibrio spp., a major menace in aquaculture industries. Vibrio spp. biofilm cause life-threatening infections in humans and animals. Forty-three marine bacterial culture supernatants were screened against the hydrophobicity index, initial attachment and biofilm formation in Vibrio spp. Twelve culture supernatants showed antibiofilm activity. The bacterial culture supernatants S8-07 (Bacillus pumilus) and S6-01 (B. indicus) inhibited the initial attachment, biofilm formation and dispersed the mature biofilm at 5% v/v concentration without inhibiting the growth. Analysis by light microscopy and confocal laser scanning microscopy showed that the architecture of the biofilm was destroyed by bacterial supernatants when compared to the control. The bacterial supernatants also reduce the surface hydrophobicity of Vibrio spp. which is one of the important requirements for biofilm formation. Further characterization of antibiofilm activity in S8-07 culture supernatant confirmed that it is an enzymatic activity and the size is more than 10 kDa and in S6-01, it is a heat-stable, non-protein compound. Furthermore, both the supernatants failed to show any biosurfactant activity. The culture supernatants of S8-07 and S6-01 with promising antibiofilm property have potential for application in medicine and marine aquaculture.
本研究旨在探讨海洋细菌培养液上清对水产养殖中主要威胁因子——弧菌属生物膜形成的影响。弧菌属生物膜可导致人类和动物的生命受到威胁的感染。我们筛选了 43 种海洋细菌培养液上清，以评估它们对弧菌属疏水性指数、初始附着和生物膜形成的影响。其中 12 种培养液上清显示出抗生物膜活性。细菌培养液上清 S8-07（巨大芽孢杆菌）和 S6-01（印度芽孢杆菌）在 5%v/v 浓度下抑制初始附着、生物膜形成并使成熟生物膜分散，而不会抑制生长。通过光学显微镜和共聚焦激光扫描显微镜分析表明，与对照组相比，细菌上清液破坏了生物膜的结构。细菌上清液还降低了弧菌属的表面疏水性，这是生物膜形成的重要要求之一。进一步对 S8-07 培养液上清液的抗生物膜活性进行表征，证实其为一种酶活性，分子量大于 10 kDa，而在 S6-01 中，它是一种热稳定的非蛋白化合物。此外，两种上清液均未显示出任何生物表面活性剂活性。具有潜在抗生物膜特性的 S8-07 和 S6-01 培养液上清液在医学和海洋水产养殖中有应用的潜力。
