Departamento de Ciencias Químicas y Farmacéuticas, Universidad Católica del Norte, Antofagasta, Chile.
J Appl Microbiol. 2008 Nov;105(5):1672-7. doi: 10.1111/j.1365-2672.2008.03878.x. Epub 2008 Oct 1.
To purify and characterize compounds with antimicrobial activity from Pseudoalteromonas haloplanktis inhibition (INH) strain.
The P. haloplanktis isolated from a scallop hatchery was used to analyse antibacterial activities. Crude extracts were obtained with ethyl acetate of the cultured broth, after separation of bacterial cells, and assays against six strains of marine bacteria and nine clinically important pathogenic bacteria. The active compounds were purified from ethyl acetate extracts, by a combination of SiO(2) column and thin layer chromatography. Two active fractions were isolated, and chemical structures of two products from the major one were unambiguously identified as isovaleric acid (3-methylbutanoic acid) and 2-methylbutyric acid (2-methylbutanoic acid), by comparing their mass spectra and (1)H- and (13)C-nuclear magnetic resonance spectra to those of authentic compounds.
In the antibacterial activity of P. haloplanktis INH strain, extra cell compounds are involucred, mainly isovaleric and 2-methylbutyric acids.
Production of antimicrobial compounds by marine micro-organisms has been widely reported; however, the efforts not always are conducted to purification and applications of these active compounds. This study is a significant contribution to the knowledge of compounds unique from marine bacteria as potential sources of new drugs in the pharmacological industry.
从抑制（INH）菌株中分离具有抗菌活性的化合物并对其进行纯化和鉴定。
从贝类孵化场分离出的假交替单胞菌被用于分析抗菌活性。在分离细菌细胞后，用乙酸乙酯从培养液中提取粗提取物，并对 6 株海洋细菌和 9 株临床重要致病菌进行了检测。从乙酸乙酯提取物中通过二氧化硅柱和薄层层析的组合分离出活性化合物。从主要的一个活性部分中分离出两个活性部分，通过比较其质谱和（1）H-和（13）C-核磁共振谱与标准化合物的谱，明确鉴定出两种产物的化学结构分别为异戊酸（3-甲基丁酸）和 2-甲基丁酸（2-甲基丁酸）。
在 INH 菌株的抗菌活性中，涉及到细胞外化合物，主要是异戊酸和 2-甲基丁酸。
海洋微生物产生抗菌化合物已被广泛报道；然而，这些活性化合物的纯化和应用并不总是被重视。本研究为海洋细菌中具有潜在药物应用价值的新型化合物的研究提供了重要的参考。
